Effect Of Dichlorobenzene On The Charreter Of Soil Enzyme

Soil enzymes and microorganisms finished soil metabolism reactions together; many scientists got the better results on soil enzyme monitoring the soil pollution by pollutants in soil ecology. Chlorobenzene is existed in environment, is one kind of pollution with more toxicity and bioaccumulation and persistence, had more harmful to the health of people and livestock. For example, the Di-chlorobenzene is listed a key substance that should be prior to monitor by EPA (Environment Protection Agency) in USA and Environmental protection bureau in China. It is less reported to study the Di-chlorobenzene ecological toxicity by soil enzyme, so there are much importance to research their influence in environment in theory and practice, finally in order to supply basis for environmental protection and remediation.By simulating method, we systematically studied the relationship between soil main hydrolyase (soil urease, invertase and alkaline phosphatase) and Di-chlorobenzene in Lou soils and red soils, and influencing factors. It is first time for us to study the enzymatic kinetic parameters in contaminated soils by Di- chlorobenzene in china, in order to understand the reaction mechanism between soil enzyme and Di-chlorobenzene. The results were as following:(1) Soil urease, invertase and alk-phosphatase could indicate the level of soil fertility.(2) With dichlorobenzene adding, urease activity of almost all soils tested were inhabited excepted several samples (middle fertility Lou soil and low fertility Red soil).(3) Soil invertase was remarkably inhabited by dichlorobenzene. Soil invertase activities showed a sharp decrease (about 34.84-65.29%)at low concentration of dichlorobenzene, then the content became slower trend. Soil invertase is more sensitive to dichlorobenzene than urease, and can be an index of soil polluted by dichlorobenzene.(4) Soil alk-phosphatase activities have the same changing law with soil invertase activity. Generally there is a little change in soil alkaline-phosphatase activities after adding pesticide.(5) soil urease Km values had such order: high-fertility soil sample>middle-fertility soil sample>low-fertility soil sample in uncontaminated soils, Vmax of middle-fertility and high-fertility samples were higher than that of low-fertility samples. When adding dichlorobenzene into soils, soil urease Km values had a little change, their Vmax increased in...