Mensurating Of Vitamin E Content And Studies On Agrobacterium-mediated Transformation Of Leymus Chinensis

Vitamin E plays an important role for human, animals and plants, whereas human and animals can't synthesize vitamin E by themselves, they relies on dietary intake. Forage grass is the main and direct resource of natural vitamin E for animals. Leymus chinensis belongs to Gramineae Leymus, it is a kind of perennial rhizome forage, which has many advantages such as high productivity, extensive adaptation, strong stress resistance, good palatability, and nutritive value. It is widely distributed in east plain of Inner-Mongolia and west plain of Northeast. In this study, content of vitamin E in Leymus chinensis was measured and analysed, receptor system of genetic transformation of Leymus chinensis was optimized, transformation mediated by agrobacterium tumefaciens was established primarily, main results obtained were following:1. The Vitamin E content of the dried leaves and different organs were measured for 6 gray-green type accessions and 12 yellow-green type accessions in Leymus chinensis by the method of fluorescence. The correlation analysis was done between Vitamin E content and seven agricultural characters, such as the yield of fresh grass, the ratio of culm / leaf, the density of plant, the plant height, the leave number of individual plant, the leaf length and the leaf width. The results indicated that the Vitamin E content ranged from 229.71μg/g to 1187.58μg/g, with the average content about 448μg/g, the average content of the gray- green type (595.78μg/g) was higher than that of the yellow-green type (375.58μg/g); the yield and vigor of the gray-green types showed a better performance than those of the yellow-green types, and the yield of fresh grass showed significant positive correlation with the plant height and the leaf length; the content of vitamin E is positive correlation with the yield of fresh grass, the density of plant, the leaf length and the leaf width, and negative correlation with the ratio of culm / leaf, the plant height and the leave number of individual plant.2. With eight genotypes of Leymus Chinesis, induction, regulation and differentiation of callus were investigated. The result showed that: the different genotypes differed significantly in the induction rate; the growth states of callus were improved effectively, embryogenic status was better and the differentiation rate was increased when subculture by turns using...