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Isolation Of Endophytic Fungi From Medicinal Plant Sabina Vulgaris Ant. And Preliminary Studies On Metabolites

Posted on:2007-02-06Degree:MasterType:Thesis
Country:ChinaCandidate:L LuFull Text:PDF
GTID:2143360185489826Subject:Pesticides
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The evergreen prostrate shrub Sabina vulgaris Ant. (syn.: Juniperus sabina Linnaeus) belonging to the genus Sabina ( family Cupressaceae) is a sort of traditional chinese herbs , which is widely distributed in the northwest of China. This studies isolated of endophytic fungi from plant Sabina vulgaris Ant. and studies on strains'metabolites. The culture were extracted with chloroform, the bioassay activity of the metabolites assayed with leaf-disk method and Leaf Dipped Method, and analysed by HPLC. The results were described as following:(1) Used the general methods. 126 strain endophytic fungi isolated from healthy organism of pharmaceutical plant Sabina vulgaris Ant. The results of the Isolation shown that the distributing was more in the veteran,and more in the stem & leafl. Culture medium include PDA and CA are becoming to isolate the endophytic fungi.(2) The concentration of the extraction from strain's fermentation were 20mg/mL. The bioassay activity against the 3rd larvae of Mythimna separate Walker. and Plutella xylostella L.were assayed with leaf-disk method and Leaf-Dipped method. The results shown that there are 28 strains had the bioassay activity against the 3rd larvae of Mythimna separate Walker., and 24 strains had the bioassay activity against the 3rd larvae of Plutella xylostella L.(3) The metabolites were analysed by HPLC. PicroPodophyllotoxin, Podophyllotoxin and Deoxypodophyllotoxn were three Standard Samples with HPLC. The result shown that there were 6 strains had the Similar tR and Characteristics of UV with the Picropodophyllotoxin, Podophyllotoxin and Deoxypodophyllotoxn.(4) The results of bioassay activity shown that the metabolites of strain LP1-3 had poison activity against 3rd larvae of Plutella xylostella L. and results HPLC shown that metabolites of strain LP1-3 had the more values tR and Characteristics of UV with the Standard Samples. Based on the bioassay activity and results with HPLC, strain LP1-3 was selected to be the continuation.(5) By isolation technique of Natural Products. One compound was obtained from the metabolites of strain LP1-3. The NO.compd A had some activities the AFC50 against the 3rd...
Keywords/Search Tags:Sabina vulgaris Ant., Endophytic fungi, Podophyllotoxin analogues, Bioachitiies Alternaria
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