The Chinese water chestnut [Eleocharis tuberose(Roxbo.)Roem. Ex. Schult] generally propagates by planting the cormels, reproductive rate is low. And it usually use small germinal disk as seeds, thus make species seriously deteriorated. In order to accelerate the purification and rejuvenation, keeping the good characteristics of the original species, it can quickly propagate good tube seedling by culturing the plant tissue in vitro, which conquer the disadvantages of normal method.In this study, we propagated Chinese water chestnut by planting the buds in vitro, the materials used in experiment were good spread in market. In order to obtain the optimum prescription for propagating the tube seedling, we search the needed nutrition for culturing the buds in vitro and the effect of various hormone matches usage on the multiplication of tube seedling and the products of tube knot bulbous.The results showed that:...
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