Studies On Wounding Signal Transduction Of P.Simonii×P.Pyramibalis C.v And Euphorbia Pulcherrima

Bio-signalling transduction is one of the exciting fields in life science. There are variously physical and/or chemical modes for communication among organisms and their cells and organs. It has been well established that volatiles plays an essential role in plant-to-plant communication when the plant is mechanically damaged or herbivored.However the study of plant to plant signal transduction has few progress. In a word, studies used woody plants as materials will be important for understanding the defense strategies of plants and providing instruction for forestry pest biological control.With thermo-desorption cold trap transfer-gas chromatography-mass spectrometry (TCT-GC-MS) device, we compared effects of some synthesized compounds on volatiles emission of poplar trees (P.Simonii×P.Pyramibalis c.v).The result show that ethylene plays an important role in interplant signal transduction mediated by Z-3-hexenal by synergizing volatile signals emission in neighboring plants. However exposed to ethylene alone did not differently induced volatile signals emission. We found that either Salicylic acid or Benzothiadiazole did not have remarkable ability induced volatile signals emission.Based on our data, healthy poplar trees exposed wounding volatiles can activate defense responses, which are involved in the jasmonate signaling pathway. The result of this, healthy poplar trees produced large amounts of volatile terpenoids and green leaf volatiles, which may be, have important function of ecophysiology. Moreover, we had timing the volatiles release, and found that the volatiles were emitted in succession. Which indicated that plant-to-plant signal transduction was conducted through time after time. Hexanal and 1-hexen-3-ol that was released at early stage of damage may be fast transfer wounding signals in interplant. This shows that previous exposure of plants to volatiles from wounding trees results in a stronger response of plants in terms of volatiles emission when their had been damaged again, which indicated poplar trees exposed wound-induced volatiles had the ability of remembering previous environmental conditions.Besides, another experiments-Euphorbia pulcherrima as a material, with method of chlorophyll fluorescence, it was found that the change of FV/FM was heterogeneity.among damaged leaves and different health leaves. To our knowledge, this is the first direct evidence that wounding signal can be transducted through acropetal and basipetal direction in damaged plants, thus plants may have multiple pathways that enable long–distance systemic wounding signal transduction.