The Research On Detection Of Newcastle Disease Virus Using Polymer Microsphere

Newcastle disease is a contagious and fatal viral disease affecting all species of birds. The Newcastle disease viruses belong to the Paramyxoviridae virus family ,and ND recognized by OIE as list A infection,which are very harmful to animals, so it is one of the most important inspection and prevention objects of all the countries all over the world . Clinical NDV is most severe in chickens , peafowl , pheasant, quail and pigeons. Turkeys may develop a milder form of the disease. Severity of disease in psittacine and passerine birds is variable. In susceptible chickens, the morbidity and mortality rates can be as high as 100 percent and 90 percent, respectively. In some species such as finches and canaries, clinical disease may not be observedPolymer microspheres are functional protein carriers . Due to they can absorb antibodies for analyte corresponding antigens, it always apply to immunoassay as diagnostic agent or ingredient of diagnostic kits.The unique properties of polymer microspheres ,which include their range of sizes , stability, uniformity, and capacity to absorb and retain fluorescent dye . Among many types of microspheres there were synthesized paticles with aldehyde, carboxyl, sulfhydryl, hydroxyl, amide, and succimide groups. All these types of microspheres were used as supports for covalent immobilization of proteins, so polymer microspheres are generally applied on melrology, denominator, biochemistry, immunomedicine, analytic chemistry, chemical industry, microeletronics area ,especially widely used in biomedicine about immunoassays, quantitation of cytokines, protein phosphorylation assays .This research is on the base of traditional sandwich ELISA,and use polymer microsphere as carriers,combination of immunofluorescence technique,flow cytometric and so on , establish a new serology method,and constrast this method to traditional sandwich ELISA about reproducibility, specificity, sensibility.This research initial established the method of detection NDV busing polymer microspheres. The specificity, sensibility of this method is the same as traditional sandwich ELISA when detect NDV. When multiple proportional dilute positive allantoic fluid of NDV to 1:1024,both methods can still...