Isolation And Culture Of Mycoplasma Mycoides Subsp.capri.and Cloning Of LppA Gene

Infectious pleuropneumonia of goats is a disease which make goats culturist feel troublesome in recent years. At clinic, diseased goats are always coughing and fever heat. Thirty to fifty percent diseased goats died of acute disease.And the other diseased goats of prolonged illness often becomes thin even loses procreative instinct. Infectious pleuropneumonia of goats was reported in Africa , Middle East, eastern Europe , the former Soviet Union , the Far East and a lot of other countries. It is one of serious goat's diseases which the mainly feeding goats countries have to face in the world. And it is considered to be the most harmful goats' disease in the world. The direct losses are high mortality , the dropped rate of producing milk or meat and that the cost of the disease control , diagnose, treat is too high.We invested the flocks of Yunyang , Beibei, Jiangjin and other areas of Chongqing. And we find thirty percent of the flocks, which quantity of cultivating is above twenty, have infected this disease. The morbidity is up to more than ninty percent in some flocks, Infectious pleuropneumonia of goats have already seriously influenced the development of feeding the meat goats in Chongqing. At present, the vaccine of Infectious pleuropneumonia of goats usually have the bad immunity result because of the difference of bacterial strain of different parts. So there is no specific drug to treat. The cost of treat are too high to influence the productivity. So it is important to study the aetiology of Infectious pleuropneumonia of goats of Chongqing. And through the study, we can make the infectious pleuropneumonia of goats die out, solve the headache problem of our goats feeding and promote the smooth development of our goats' industrialization.The gene of LppA is a kind of surface antigenic gene of membrane in Mycoplasma mycoides subsp.capri. The LppA gene start from 251 base to 1822 base in Mycoplasma mycoides subsp. capri. DNA sequence. And the protein of LppA is the important parts of antigen of Mycoplasma mycoides subsp. capri. At this dissertation, the study for LppA aim at laid a foundation to find more further vaccine of nucleic acid.First of all, we dissect the goats which suffering Infectious pleuropneumonia of goats in the flocks of Chongqing Beibei Tianfu town. Than we separate the pathogeny with the improved culture...