Study On The Primary Identification Of 8 Bacteria Isolates From Rot Potato Tuber Via 16S RDNA, Pathogenic Test And Indoors Control

As a crop used as both vegetable and grain , potato play an important role in people's life. But potato is suffering yield and quality reducing these years because of diseases caused by microbiology ,among them bacteria are main pathogen.Diseased samples were collected from Ding Xi, Hui Chuan, Wei Yuan, Zhang Ye where potato are the third important crop and eight isolates of bacteria were acquired by lineation on flat and isolation. Pathogenic test were done through two methods. Through the electron microscope, the isolates No.4 and No.7 are bacillus shaped .After being inoculated to potatoes by different methods , eight bacteria might cause sick symptom of different diversity with different isolates. Potatoes inoculated with bacterial isolates were planted in vermiculite. The different bacteria may cause potatoe reduction of out put. The most important isolate is No. 7; another important one was No.8.After extract DNA from the eight bacteria, PCR of 16S rDNA experiments were done with bacterial specific primer and the primary identification result were gotten: Erwinia persicinus, Pantoea sp, Microbacterium sp, Microbacterium sp, Pseudomonas sp., Erwinia persicinus, Pseudomonas sp.。The inhibition of chitosan of different concentration, H₂O₂ solution and different bactericide were done in laborary, the best effect of chitosan concentration was 2.0².5%. The effect of H₂O₂ to the eight bacteria became better with the H₂O₂ concentration increasing. Among the three bactericides, one had clear effect to inhibit bacteria and the effect became better with the concentration increasing, while others had no effect.