Optimization Of SSR And RAPD Analysis System Of Tomato And Identification Of The SCAR Marker For The TMV Resistance Gene TM-2～(nv) In Tomato (Lycopersicon Esculentum)

Tomato (Lycopersicon esculentum Miller) is one of the worldwide growing vegetables. And is possessed of consequence in the economy of agriculture and agriculture market. For its idiographic sapor, abundant alimentation, fresh color and peculiar medical value, it attracts more and more consumers. The total yearly yield of the tomato has reached more than 80 million tons. But tomato's production has been restricted by some diseases, among them, TMV, CMV, are the main diseases in tomato. The most effective method to control these diseases is to screen and to breed resistant varieties. By identifying the molecular markers for resistant genes to rust and wilt and screening resistant germplasm in tomato, we hope to set up a breeding procedure for maker-assisted selection and to get more resistant germplasm for tomato breeding program. The major results in this paper are as follows:1 Optimization of Simple Sequence Repeat (SSR) technique in TomatoWith the meterials of tomato, a protocol of reproducible simple sequence repeat (SSR) was established for tomato. The main factors influencing SSR-PCR including the concentration of Mg2+,dNTPs, Taq polymerase, annealing temperature and the cycles were investigated.Establish the techiqu system that was suit to the tomato : 10×PCR Buffer,Mg²⁺ 3.0mmol·L ^-1,dNTPs 0.5 mmol·L^-1,Taq DNA polymerase 0.05 U·μL^-1,annealing temperature 58℃, numbers of cycles 35。 2 Optimization on Random Amplified Polymorphism DNA(RAPD)analysis system of TomatoWith the meterials of tomato, a protocol of reproducible simple sequence repeat (SSR) was established for tomato. The main factors influencing SSR-PCR including the concentration of Mg2+,dNTPs, Taq polymerase, annealing temperature and the cycles were investigated.Establish the techiqu system that was suit to the tomato : 10×PCR Buffer,Mg²⁺ 3.0mmol·L^-1,dNTPs 0.5 mmol·L^-1,Taq DNA polymerase 0.05 U·μL^-1,annealing temperature 37℃, numbers of cycles 45。3 Identification of molecular markers for the TMV resistance gene Tm-2^nv in tomatoSCAR analysis of the TMV resistance materials of tomato, and the amplification showed that, this SCAR marker was the specificity for the Tm-2^nv gene. The length of the tide was about 700bp, and using this marker, we could expediently identify and select the TMV resistance materials of tomato.