Preliminary Estabilishment Of Photoautotrophic Culture System In Vitro Cymbidium And Phalaenopsis Shoots

Cymbidium and Phalaenopsis in vitro shoots cultured in novel enveloped-shaped vessels, the Culture Pack (CP), made of a fluorocarbon polymer film which possesses gas permeability, light transmittance, thermal stability and heat seal process ability were used as plant materials for experiments to investigate the vigorous development and growth of in vitro shoots respectively under normal culture, light-emitting diode (LED) radiation resource and CO₂ enrichment. The results showed:(1) Under normal culture, the plantlets growth of Cymbidium grown in the culture pack rockwool system (CP·RW) was better than CP·A and FL·A, while the plantlets growth of Phalaenopsis grown in CP·A and FL·A was significantly better than in CP·RW. Compared with the normal culture, the plantlets growth of Cymbidium and Phalaenopsis obtained in the culture vessel (CP) have significant improvement under CO₂ enrichment.(2) Under the improvement of CO₂ concentration in the culture vessel (CP), compared with standard light intensity (1700 Lx), the high light intensity (3000 Lx) could significantly promote the plantlets growth and increase the leaf stomata density of Cymbidium. As for the plantlets growth of Phalaenopsis, increaseing light intensity had no significant improvement; but high light intensity (3500 Lx) could significantly increase the leaf stomata density and area of in vitro Phalaenopsis shoots. Under CO₂ enrichment, the plantlets growth of Cymbidium and Phalaenopsis cultured in the sugar-free culture medium could grow normally by photoautotrophic capability, but a bit slowly. Increasing the sucrose concentration in the culture medium could significantly promote the plantlets growth of Cymbidium and Phalaenopsis. The optimal sucrose concentration is 1% for the plantlets growth of Phalaenopsis and 2% for the plantlets growth of Cymbidium.(3) Under normal culture and CO₂ enrichment, compared with the control...