Evaluating On Phenotype Diversity And Construction Of Primary Core Collection Of Grape Germplasm

China is one of the major gene centers of Vitis species origination. For incomplete statistics, China have collected and preserved more than 1500 grape varieties in resources nursery, the number of the germplasm is very large. 950 germplasm growing in grape resources nursery of'Zhengzhou Fruit Tree Research Institute of CAAS'were used as materials, primary core collection of grape were established based on the phenotype diversity by using the DPS3.01 software.1. Main component analysis of phenotype characters was conducted and it showed that the top fifteen components explained 65.23% information. Tn terms of contribution of characters, the anthocyanin distribution of the tip of young shoot, prostrate hairs density on internodes of shoot, anthocyanin colouration intensity of young leaf, number of lobes of mature leaf, shape of the base of upper sinuses of mature leaf, profile of mature leaf, blistering of the upper side, shape of bunch, shape and color of berry etc showed the difference between cultivars better than the other characters.2. Thirty-two major characters were graded based on the distribution analysis and a model was selected for each grade. Most of the shape of blade of mature leaf are pentagonal and wedge-shaped, the shape of petiole sinus are semi-wide open and slightly-open, tooth at petiole sinus are absent, shape and color of berry are round and green-yellow.3. The shannon-weaver indexes by one layer was calculated in this paper, each region has different genetic diversity index of comprehensive characters and each character has difference in different group. Results and orders of each region were V.Vinifera.> Relatives of V.labrusca> Seibel>Kyoho.4. All the germplasm were divided into 9 regions according to germplasm types, each region sampled by square root strategy with sampling ratio of 15%,20%,25% and 30%, respectively obtained 124, 170, 205and 252 germplasm. Two layers and gradually cluster analysis sampled by the same strategy and ratio, obtained the same quantities germplasm. 5. The representative of primary core collection was also tested by using genetic diversity index, reserving phenotypes of characters and distribution frequency of qualitive characters. The results indicated that, the core collection assembled by the ratio of 15% very likely represents the great majority of the genotypes and genetic diversity of original populations.6. The wild grape and rootstocks were specially treated, respectively selected 9 germplasm into primary core collection. Added cluster sampling at the ratio of 15%, finally the core collection including 142 cultivars of grape was established.The shannon-weaver indexs and constructed the primary core collection of grpe was analyzed based on the phenotype types of grape, it also has reference function for other fruit trees.