Cloning And Function Analysis MgGLK1,MgMFS1 And MgHAP Genes Related To Pathogen Infection Of Magnaporthe Oryzae

As the causal agent of rice blast fungus,Magnaporthe oryzae,is considered a model organism for studing the interaction between host plant and fungal pathogen.The reserch about its pathogenesis is not olny contribute to the scientific precaution of the rice blast fungal but also have signification to the pathogenesis of other fungus.Here the report demensrates three specific expression genes related to infecton of Magnaporthe oryzae,which koncked out and over-expressed respectively.Through out the analysis of the mutants to determine the role that they played in the initial infection process of M. grisea.MGG₁0005.6 and MGG₀1877.6 were high expression genes and MGG₀5058.6 is low expression genes during fungal infection.The gene deletion mutants were obtained.Named MgGLK1,MgMFS11 and MgHAP respectively.The phenotype analysis results showed that compared to wild-type strain ,the sporulation of MgGLK1 reduced;the conidial germination and appressorium formation of MgGLK1 were slightly delayed;There is no no difference in the pathogenicity between the mutants and wild-type strain.About the mutant of MgMFS1,the results shows that the the growth rate, sporulation, conidial germination and appressorium morphology of knockout mutant strain were not affected.Compared with wild-type,MgHAP deletion mutants'sporulation decreased and conidial germination and appressorium formation were slightly delayed.The analysis of overexpression of mutants MgHAP found that the sporulation , appressorium formation and pathogenicity were not significantly different.In summary,MgGLK1,and MgHAP may be involved in control of sporulation and spore germination of the rice blast fungus,and play a role in the infection process,but they are not essential for pathogenicity.