| H5 subtype Avian Influenza not only threats to poultry industry, but also causes huge economic losses all over the world in recent years.The growth characteristics of broilers are short growth cycle, rapid growth-up and most nutrients of feed turned into muscle growth. .So Broiler parts of the body are burdened and inside always in the state of stress reaction. As a result, broilers are poorer ability to resist avian flu. These cause more significant loss in broiler breeding industry in many countries and regions. The research on the time of maternal antibody against challenge, different cycles of concentration antigen of Inactivated oil-emulsion vaccines, immune effect of different vaccine adjuvant, effect of combined inactivated and live vaccine was studied and cellular immune response was detected, to find out satisfactory vaccines against Avian Influenza and provide scientific basis on prevention and control for broiler chickens Avian Influenza.Dynamic of maternal antibody and virus attack protection at different time was studied to observe the challenge time of maternal antibody by choosing a number of local broilers in Haerbin. The result is that HI antibody titers is 4log2 for one-day broiler and decline during the next week and is below 3log2 seven days later. The mortality is 12/20 and total shedding is 18/20. with 3-days vaccinated broilers, but 16/20 with7-days old and total shedding is 20/20. The mortality is up to 19/20 with 14-days vaccinated broilers.About 50 days old broiler will sell on the market, but they need some off-drug period for food safety. Based on experiment result, the broilers should be about 7 days old.By comparison of immune effect between different multiple cycles of concentration antigen of Inactivated oil-emulsion vaccines and (Re-5 Strain) Inactivated oil-emulsion vaccines, it shows that (Re-3 Strain) 3 cycles of concentration antigen of Inactivated oil-emulsion vaccines and (Re-5 Strain) 5 cycles of concentration antigen of Inactivated oil-emulsion vaccines have much better effect than (Re-5 Strain) Inactivated oil-emulsion vaccines. 0ne to six weeks later, HI antibody of different multiple cycles of concentration antigen is higher than none concentration antigen. HI antibody of 5 cycles of concentration antigen of inactivated oil-emulsion vaccines has maximum value 6.3log2, 3 cycles of concentration antigen of inactivated oil-emulsion vaccines 5.6log2 and none concentration antigen is 3.6log2. It turns out that increase antigenic content can improve immune reaction of avian influenza vaccine.4 (Re-5 Strain) different vaccine adjuvant inactivated vaccines have been developed to select desired adjuvant and compare the immune effect. (Re-5 Strain) Montanide ISA71VG Inactivated oil-emulsion vaccines Vaccinated one to six weeks later, HI antibody increases and up to 4.7log2 at the sixth week. Virus attack at two weeks later, no virus shedding, clinical signs, or deaths were observed. According to antibody level and detoxification, (Re-5 Strain) Montanide ISA71VG Inactivated oil-emulsion vaccines has better application prospect, compared with traditional Inactivated oil-emulsion vaccine,Montanide ISA206VG Inactivated oil-emulsion vaccine and aluminium adjuvant Inactivated oil-emulsion vaccine.Immune effect about vaccinated broilers was researched by using combined vaccines. It figures out that (Re-5 Strain) Montanide ISA71VG Inactivated oil-emulsion vaccines,Traditional (Re-5 Strain) Inactivated oil-emulsion vaccines combined with Avian Influenza Recombinant Newcastle Disease virus bivalent vaccine,Live(rL-H5 Strain) vaccinated at the same time or one-week interval, HI antibody are higher than none combined group after one to six weeks. Furthermore in virus attacking test at different time point, combined vaccines can provide completely immune protection of virus attack without detoxification or deaths. The results show that combined immune of inactivated vaccine against avain influenza and live vaccine triggers satisfactory humoral immune response. The research provides reference for immunization schedule of inactivated and live vaccines.In this paper cellular immune response was studied by 4 different kinds of H5 Subtype Vaccines against Avian Influenza. It figures out that while DNA vaccines , Avian Influenza Recombinant Newcastle Disease virus bivalent vaccine, Live(rL-H5 Strain),(Re-5 Strain)Montanide ISA71VG Inactivated oil-emulsion vaccines, (Re-5 Strain) Inactivated oil-emulsion vaccines immune chickens the relative percent contents of CD4+ and CD8+ subsets, CD3+ T lymphocytes in periphery blood, spleen and thymus of immunized groups are higher than the unvaccinated groups, and the relative percent contents of CD4+ T lymphocytes in spleen consistently increased after vaccination. Test result of SI-value and NO secretory volume of macrophages in the different stage display that immunized groups higher than the control groups (p<0.05), selected vaccine can stimulate strong cellular immune response with AA broiler. |
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