Molecular Phylogenies Study Of Rana Animals And DNA Fingerprinting Application In The Discrimination Of Oviductus Ranae

Cordyce Pssinensis (Berk) Sace is a famous and Preeious Chinese traditional medieine which is one of the top three valuable tonic together with Radix Ginseng and Cornu Cervi Pantotrichum.A large number of existing researches showed that the anamorph of Cordyce Pssinensis is Hirsutella sinensis. In this study, the culture of Anamorph of Cordyce Pssinensis,vasdiseussed beeause of its expense and rare resources, while the Cordyeepin in fermenied myeelia and natural Cordycops sinensis was tested. We also tested the infeetion of mycelia on hepialidae larvae and discussed on the comprehensive utilization of waste solution afte rliquid statefermeniation.The results of the nitrogen souree experiment showed that has best absorption and utilization on pupa of T.Weeanseethatnitrogen is the esseniiallivingelementsofHirsutellasinensis, andtheutilizationratioonanimalnitrogenOptimum conditions of preparation and reproduction of lactococcus lactis'protoplast were studied. After adding 0.6ug/ml ampicillin for pre-treatment during appropriate growing season of lactococcus lactis,selecting 6mg/ml enzyme solution and enzymolysis for 30min at 37℃, the highest product of production rate and reproduction rate can reach 46%. Mutagenesis of protoplast was conducted by ultraviolet radiation.HU17 was screened through agar block method and agar diffusion method.The genetic stability of HU17 was proven to be better through genetic stability test.After five generations,the potency of nisin was still around 3200IU/ml.Therefore,HU17 was screened as the lipstatin of nisin through ultraviolet mutagenesis.Concentration of corn steep liquor,corn mash,KH2PO4 and NaCl which affect the potency of nisin was investigated through one-factor experiment.The results showed that the ratio of the volume of corn steep liquor and corn mash was 8:2,the optimum concentration of KH2PO4 and NaCl were 14g/L and 2g/L.Concentration of corn steep liquor,corn mash,KH2PO4 and NaCl was optimized through Box-Behnken method and response surface methodologyrsm to show that the optimum components of fermention medium of nisin were:corn steep liquor:0.88L/L,corn mash:0.12L/L, KH2PO4:15.6g/L, NaCl:2.5g/L.The potency of nisin was 5590IU/ml under optimum conditions.Scale-up cultivation of HU17 was performed in 5L fermentor.Effects of the inoculum age, inoculum dose.dissolved oxygen and initial PH of the medium on the potency and biomass of nisin were studied.The results showed that the potency and biomass of nisin were 6200IU/ml and 1.45g/L when the optimum inoculum age was 8h.the optimum inoculum dose was 3%.the optimum dissolved oxygen was 40%.initial PH of the medium was 7.