| Thirteen similar body condition and 15 day-old age Altay sheep were selected to sheep pen, then adopt to the method of artificial milk until 30 day-old age. Feed them different nutrient levels of diets on different growth stages. Days were conducted in sheep 30,60,90,120,150,180 old age to get blood from the left jugular vein.Serum was separated for blood physiological and biochemical indexes; measured and record the development level of lipid tail (tail length, tail width, tail height); get the tail fat and legs muscle, about 0.5g. Use RT-PCR method to detect the expression of adrenergic receptor gene, and analysis the correlation between developmental level of tail and expression of adrenergic receptor gene. The results of serum biochemical indexes showed:there was no significant difference in the blood biochemical indexes at the age of 30,60,90,120,150,180 on Altay sheep. Total cholesterol, low density lipoprotein, high density lipoprotein levels were highest in the 30 days of age,31-90 days to increase their levels gradually decreased with age, but in the 91-120 days of age but also on the rise,121-180 days decreased gradually; triglyceride and urea nitrogen levels were lowest in the 30 age,31-90 showed a trend of gradually increasing and reached the highest levels in the 60-90 age,91-180 days old slight ups and downs, but overall continued to show a downward trend. Aspartate aminotransferase, lactate dehydrogenase and alanine aminotransferase levels were lowest in the 30 days of age, aspartate aminotransferase levels highest in 31-60 days; lactate dehydrogenase content of the highest in the 90-120 days of age; alanine aminotransferase The content of the highest levels of 120-150 days, then remained relatively stable.Uropygial measurement data show that:the development of high-end and tail length variation is similar to the increases are between the 0.65-1.99cm/30d and 0.73-1.97cm/30d.30-60 days the biggest increase in two stages, in the 60-120 day period, tail height and tail length growth rates have gradually decreased with increasing age; 120-150 days of age, have increased since then growth was downward trend. Increase in tail width than the tail length, tail tall, as 0.78-2.81cm/30d.120 days ago, the end is the growth rate decreased with increasing age; 120-150 days of age, an increase of rapid increase, and the maximum for 2.81cm/30d, growth and decline thereafter. Quantitative results showed that the genes:the muscle tissue ofβ1AR,β2-AR,β3-AR and a2A-AR gene expression in the sheep, the level at 30 days than lower, then increase rapidly. At 60-150 days of age, to detect the genetic relationship of the abundance of a2A-AR>β2-AR>β1-AR>β3-AR, but 180 days of age in the sheep, the abundance wasβ2-AR>a2A-AR>β1-AR>β3-AR. Adipose tissue in theβ1-AR,β2-AR,β3-AR and a2A-AR gene expression at 30 days in sheep have the highest level, when testing the relationship between the abundance of the gene for theβ2-AR>a2A-AR>β1-AR>β3-AR. Since then the gene expression level increased with age generally decreased, but theβ3-AR gene in 120 days of age,β2-AR and a2A:AR gene in 180 days of age, there are increased levels of its expression. Uropygial adrenergic receptor development levels and correlation of gene expression levels:with increasing age in sheep muscle tissue levels ofβ2-AR gene expression and sheep tail length, tail width, tail high level of development was a significant positive related;β1-AR,β3-AR gene expression and sheep tail length, tail width, tail high level of development was significantly positively correlated; a2A-AR gene expression and development of tail fat was no significant correlation. Adipose tissue,β3-AR gene expression levels were significantly negatively correlated with the development levels of tail length and tail. The expression of a2A-AR and B2-AR gene was no significant correlation with the development of tail adipose.
|
PDF Full Text Request