Gene Clonging And Functional Analysis Of Pg13 In Rice (Orvza Sativa L.)

As a model plant for the study of monocotyledon plants, rice is the staple food for more than half of the population in the world and also one of the most important food crops in China. Leaf is an important organ for photosynthesis and plays a significant role in rice growth and development. Leaf color mutation is a class of very obvious trait mutation, and they are of great significance in the study of the photosynthetic system, structure, function and regulation of higher plants. So far, at least 79 mutations related to leaf color have been targeted in rice, and also a number of genes have been cloned.Pgl3(pale-green-leaf) in this study was from the laboratory by EMS mutagenesis of rice variety Nipponbare offspring. Map-based cloning method and the genome sequence analysis showed that PGL3 was mapped on chromosome 10, and the mutation was a frameshift mutation which caused by one base-pair insertion. Compared to the wild-type, the chlorophyll content of chlorophyll a, chlorophyll b and carotenoids were significantly declined in the mutant, the chloroplast grana lamellae were also significantly reduced by transmission electron microscopy analysis. Tissue specificity expression of the gene revealed that PGL3 was expressed in rice roots, stems, leaves, sheath and spike. Meanwhile, complementary vector, GFP expression vector, GUS vector, over-expression and interference vector, were also constructed, and then introduced into rice calli by agrobacterium tumefaciens-mediated transformation. The functional complementation of transgenosis test, indicated that PGL3 was the target gene which controls pgl3 mutant phenotype.