The Interaction Between Trichinella Spiralis And Intestinal Microbes

The human body contains a variety of microorganisms, even if small but significant portion of humanity. Mucosa constantly exposed to various antigens and molecular environment, these molecules derived from exogenous antigen and a variety of intestinal sojourn food or symbiotic bacteria. These symbiotic bacteria at least 400 species. Per gram of intestinal contents in about 1012 bacteria. In fact, humans and their symbiotic bacteria has developed mutually beneficial symbiotic relationship, normal intestinal bacteria can not only promote development, but also the United lymphoid tissue to promote digestion and prevent pathogen invasion.May be parasitic Trichinella spiralis muscle and other tissues in the human, and human evolution are closely related. Large amounts of data have been studied thoroughly the relationship between parasite and host.The relationship between intestinal bacteria and parasites have become the research hotspot in recent years. Symbiotic bacteria and intestinal parasites of the gastrointestinal tract and the entire dwelling intestinal microbial flora associated with the evolution. The relationship between bacteria and parasites confirms the existing data, such as filarial and Wolbachia, however, microorganisms and parasites in the symbiotic relationship between the non-functional after research. This complex of intestinal inflammation in the gut microbiology and autoimmune diseases, immune regulation mechanism plays a very important significance.In addition to bacteria, fungi within the host may also affect the survival of intestinal parasites, its impact is like? No data interpretation process. The interaction between microorganisms and parasites of the gut leads to a new dynamic research on the expansion of the existing intestinal homeostasis and adjust the concept of immunity has far-reaching significance.We found:bacteria, fungi, and the role of between Trichinella spiralis, in which the relationship is this:when a single culture group Trichinella spiralis, Trichinella spiralis and EHEC EDL933 cocultured group, Trichinella spiralis incubated with E. coli DH5a group of insect larvae incubation rate comparison, Escherichia coli group of larvae of Trichinella spiralis worm yield 0.186275 0.84 compared with negative control group significantly reduced, while the output of E. coli DH5a group was 1.641173 and the negative control group of larvae of the Trichinella spiralis worm no significant effect on yield. When cultured alone group Trichinella spiralis, Trichinella spiralis and co-cultured with Lactobacillus bulgaricus, Lactobacillus acidophilus Trichinella spiralis and incubated rate between co-culture group, due to the impact of Lactobacillus, two groups of insect larvae Lactobacillus group compared with negative yield 0.84 in control group had significantly higher yield, Lactobacillus bulgaricus and Lactobacillus acidophilus and output rates were 3.448329,2.223532. When cultured alone group Trichinella spiralis, Trichinella spiralis and incubated rate of the yeast culture group were compared, the yeast group with the output rate of 1.270387 to 0.84 comparing negative control group, due to the impact of yeast insect larvae than the negative control group yield a certain increased.The initial phase of Trichinella spiralis adult training adults in good condition under the microscope and vigorous activity, there is no insect larvae production. After 24h culture under the microscope the movement of adult and larval state of production, including Trichinella spiralis cultured alone in the negative control group, normal activities of Trichinella spiralis adults, there is a certain insect larvae production, swimming activity. Trichinella spiralis and Escherichia coli were cultured adult group activities Trichinella spiralis curled slowly into shape, have less output and activities of newborn larvae slowly. Trichinella spiralis were cultured with E. coli DH5a normal activities of the group and adult Trichinella spiralis little active swimming, which produce newborn larvae and normal activities. Trichinella spiralis and Lactobacillus bulgaricus and Lactobacillus acidophilus group co-culture of adult Trichinella spiralis strong and swimming activities active, more active newborn larvae and the movement, Lactobacillus bulgaricus, Lactobacillus acidophilus group than in the volume of production insects slightly. Co-culture with the yeast Trichinella spiralis adult nonnal activities of the group and activist, including newborn larvae and activist.Transwell culture bacteria Trichinella spiralis, trichinellosis and germs NO concentration in culture supernatants were compared, the bacteria are not in direct contact with the group and the control group Trichinella spiralis NO concentrations are similar, there is no more substantial changes. Direct contact with the bacteria Trichinella spiralis group a relatively large change in NO concentration. Including Escherichia coli were cultured with Trichinella spiralis NO change in the culture medium in a larger group, NO co-culture of Salmonella have some changes in co-culture of Lactobacillus acidophilus group, there are NO changes in the other group did not change significantly. At different time points of Trichinella spiralis infection in different bacteria will vary the amount of NO. These results can be seen in the bacterial co-culture with Trichinella spiralis NO change in the culture medium when the rate was significantly, and the bacteria cultured with Trichinella spiralis no contact NO concentration in the culture medium did not change significantly. Possible contact with cultured bacteria and Trichinella spiralis, Trichinella spiralis bacteria can promote the role of certain signal transduction, and thus the health of Trichinella spiralis have some influence, while larvae of Trichinella spiralis production rate to some extent.Through this experiment, we found that harmful bacteria Escherichia coli intestinal Trichinella larvae of T. spiralis adult worm output and the health status of a certain extent, the intestinal bacteria, Lactobacillus bulgaricus and Lactobacillus acidophilus The larvae of Trichinella spiralis adult worm output and the health status of a facilitating role.Further, we studied the infection of Trichinella spiralis in SD rats, the pathological changes. Trichinella spiralis infection 7 days,14 days,40 days for SD rats and not infected with the SD rats diaphragm, duodenum, jejunum, ileum, colon by pathological fixed, embedded, sliced, HE staining and observed under light microscope set, showing the various organizations at different times of the pathological changes, which further confirmed that the life history of Trichinella spiralis. SD rats in each period to take the blood of the supernatant, using ELISA method for detection of cytokines. IL-12 levels in the early stages of infection than the negative control group, to the late stage of infection IL-12 levels close to normal. IL-4 content in time with the infection of IL-4 levels showed an increasing tendency. The results confirmed that type 1 cytokine response mediated by the host of the sensitivity of Trichinella spiralis infection, and 2 type cytokine response is the host for the intestinal parasite Trichinella spiralis immunity necessary.Here, we study the hatching rate and NO changes in Trichinella spiralis driving mechanism of bacteria, bacterial infection mechanisms and morphological changes Trichinella spiralis mammalian host immune response mechanisms. Further expansion of the now known intestinal homeostasis and immune function regulation concept.