| Acyltransferase is a large family of multifunctional proteins with diversity chemistry functions. One of the main features is the catalytic protein acylation and de-acylation. N-acetyltransferase (N-acetyltransferase, NAT) catalyzes the acetyl transfer from acetyl-CoA on the nitrogen atom to isoniazid. Histone acetyltransferase enzyme (HAT) and histone deacetylase (histone deacetylase, HDAC) catalyzed histone acetylation and deacetylation on N-terminal residues respectively, which is also the common post-translation regulation mechanism in eukaryotic. So in a sense, acyltransferase have the biochemistry regulation function to activate protein biological activity, to regulate gene expression and other features. But in the body, with a variety of acyltransferase, in addition to acylation and catalytic proteins to acyl barbarian, but also play other important functions, such as cholesterol esterification catalyzed by acyl coenzyme A: cholesterol acyltransferase (ACAT), which plays an vital role to keep the balance for cholesterol metabolism whether in the cell or in individual level.The purpose of this study is the isolation and cloning of homologous sequences in rice were identified to BAHD acyltransferase gene. Then by using of both over-expression and/or RNA interference (RNAi) technology to study the function of these candidate genes in rice secondary metabolism, biotic stress and abiotic stress. Further we target to investigate the mechanism for those genes which involved in biotic stress and abiotic stress.Detailed results are as follows:1. The full-length cDNA of Os02g39850, Os04g42250, Os06g08640, Os09g25460 and Os10g35950 were cloned. The recombinant vector for RNAi andoverexpression were designated as ds1301: Os02g39850, pU1301: Os04g42250, ds1301: Os04g42250 and ds1301: Os06g08640. Then the recombinant plant expression vectors were directly transferred into Agrobacterium tumefaciens stain EHA105.2. With the Agrobacterium-mediated transformation system, the plant expression vector ds1301: Os02g39850, pU1301: Os04g42250, ds1301: Os04g42250 and ds1301: Os06g08640 were introduced into the rice. Currently, 6 and 17 transgenic lines were obtained for pU1301: Os04g42250 and ds1301: Os04g42250 from rice donor ZH11, while they presented the positive ratio of 75% and 44.7% respectively.3. All the transgenic lines for Os04g42250 were inoculated with PXO99. Disease symptom were investigated after 10 day of inoculation. There have 7 lines of ds1301: Os04g42250 shown more susceptible to PXO99, the average lesion length was 9.0±1.2 cm, while the average lesion length of the control wild-typeZH11 was 5.0±0.6 cm. For pU1301: Os04g42250 transgenic lines, there have 4 lines enhanced the resistance to PXO99, the average lesion length is limited to 1.3±0.3 cm. These results suggested that Os04g42250 was involved into plant disease resistance in rice. When Os04g42250 gene silencing, the plants were more susceptible, while Os04g42250 genes was overexpressed in rice, the resistance of plants was increased.Bioflavonoids have a variety of biological activity, can be used for anti-aging, treatment and prevention of cancer, cardiovascular disease and other diseases. So it is handle great value to develop and application. The current approach to meet the market demand is mainly from natural materials by chemical methods such as ginkgo, fruit extracts, and its high cost and low content. This study will focus on genetic modification of cherry tomato with combinating with fruit specific promoter E8, flavonoids regulation gene AtMYB12 and plant marker-free transformation vector pX6-GFP, to achieve cherry tomato transgenic lines enriched flavonoids only in fruit. This tomato will highly enriched flavonoids (quercetin and rutin) and caffeoyl quinic acid in the fruit which will be the best candidate for industrial production with economic values.
|
PDF Full Text Request