| Cotton is one of the most important economic crops in our country, verticillium wilt of cotton is a vascular disease mainly caused by the soil-born filamentous fungus Verticillium dahliae and gives serious wilt phenotype for cotton and results in severe loss of cotton production as well as downgrade of fiber quality. At present few remarkable research progress was made in the control and breeding against the pathogen. In this study, to study the mechanisms associated with defense responses in wilt-resistant sea-island cotton (Gossypium barbadense) upon V. dahliae infection, a comparative proteomic analysis between infected and mock-inoculated roots was performed by 2-DE combined with local EST database-assisted MS/MS analysis. The results are as follows:1,A total of 51 up-regulated and 18 down-regulated proteins were identified from G. barbadense var. Hai 7124, a cultivar showing resistance against V. dahliae. These proteins are mainly involved in defense and stress responses, primary and secondary metabolisms, lipid transport and cytoskeleton reorganization.2,Previous study identified a pathogen-responsive chitinase (named GbCHI) from sea-island cotton (Gossypium barbadense) by comparative proteomics. The GbCHI cDNA was cloned3,The anti-fungus function of the gene products was investigated. qRT-PCR analysis indicated that GbCHI was expressed constitutively in root, stem, leaf, flower and ovule of cotton plant.4,the expression could be induced by Verticillium dahliae, SA, ACC and JA hormones. Subcellular localization analysis using GFP-tagged proteins showed that GbCHI-GFP fusion proteins were targeted mainly to the plasma membrane.5,Anti-fungal assay demonstrated that GbCHI could inhibit spore germination and hyphae growth of Verticillium dahliae significantly. Together, these results provide important information for understanding the cellular function of GbCHI and for exploring the application potential of the gene in molecular breeding of wilt-tolerant cotton plants. |
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