Studied On Propagation And Salt Resistance Of Dichroa Febrifuga L.

Dichroa febrifuga Lour,a kind of deciduous shrub in wildness belong to the family of Hydrangeaceae and Dichroa genus which is so valuable resources that should be protect in all kind method. In order to protect and put it in use in garden and meet the material requirement of landscape application, the seed germination, cutting propagation, tissue culture and NaCl stress were researched,and the suggestions were given for protection, utilization and domestication of these wild ornamental and herb trees.The results shown as follow:1. The seeds were collected in Jan.2008, Jan.2009 and Jan.2010, respectively,that were planted in four media (garden soil,sand,1/2 sand+1/2peat,1/3 sand+1/3 peat+1/3 vermiculite) for germination test. The results shown that the highest germination percentage was the one which has been collected in Jun.2010 which were stored in a refrigerator with 4℃. Then is the one that collected in Jun. 2009. Germinating percentage was zero from which the seeds collected in Jun.2008. The results indicated that seed of Dichroa febrifuga are not resistant to store for which there were none germination had stored for 2 years, and the longer time of storege the lower germination percentage in the condition of indoor temperature. All of the index of germination percentage(abbr.GP),germination potential(GP), germination index(GI) and vigour index(VI)which were stored in refrigerator were higher than that were stored in the condition of indoor temperature. And All of the index of GP, GPT, GI and VI which were stored in the storage practice of bacca were higher than that were stored in bare seed. The research of media in germination indicted that the GP,GPT,GI and VI in the medium of sand were higher than that in both of the medium of Sand+Pea and Sand+Peat+Vermiculite medium,and the result indicted that the seed of Dichroa febrifuga Lour was light-requiring seed, the highest germination percentage was obtain by stored in refrigerator for a short time and in the condition of illumination.2. Cutting propagation research including cutting season, cutting media, soft and hard branch cutting as well as well as hormone concentrations were studied and the survival rate, leaf number, rooting number, root vitality, root effect index were tested.. The results shown that hard branch was soaked by 500(mg/L) NAA for 30s, and planted in medium of 50% river sand mixed with 50% peat in autumn had the highest survival rate for which was eighty four percent. The results of cutting research in spring shown that survival rate of soft branches cutting was a hundred percent that were planted in the medium of 50% river sand mixed with 50% peat and sand mixed with peat and vermiculite with the proportion of 1:1:1, for which the cuttings were soaked by NNA(500mg/L) with 60 seconds.The survival rate was a hundred percent that the cuttings were soaked in NAA(1000mg/L) with 30 seconds in the medium of pure river sand.The research demonstrated that it was comprehensive root type that mainly of root primordium initialed from cortex, and auxiliary root primordium was initialed from callus and the root effect index related to leaf number and rooting number. Both the hard cuttings in autumn and soft cuttings in spring had good results, respectively.3. Semi-lignified shoots were selected from Dichroa febrifuga Lour which used as explant in tissues culture experiment. The results indicted the effectively sterilization were that the explant disinfected with 75% alcohol for 25 second then washing it three times with sterile water,and then soaked in 0.1% mercuric chloride for 10minutes,and there was none contaminated in medium of tissue culture.The medium of MS medium+6-BA 2mg/L+NAA0.1mg/L+agar 5g/L+sucrose 20g/L as Dichroa febrifuga Lour was used as stem bud induction, and MS medium+ IBA 0.1 mg/L+GA3 1 mg/L+agar 5g/L+sucrose 20g/L was used as the induction medium to promote the differentiation of root stem. Dichroa febrifuga Lour's stem and root buds differentiation induction effect was obvious when pH value of 5.8～6.0.4. Salt stress mechanism was studied by NaCl(0,200,400,800 and 1200mg/L) of Dichroa febrifuga Lour, and the results shown that low concentrations of NaCl can promote the growth and development of Dichroa febrifuga Lour among which 800(mg/ L) was suitable concentration to promote the growth and development. As the NaCl concentration rising, cell membrane damaged rate increased gradually, and malondialdehyde (MDA) contend increased in lower NaCl stress treatment and then decreased significantly in higher concentration of NaCl treat. MDA concentration reached the highest when NaCl contend was 400(mg/L).The most activity of Superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) was that the NaCl contend was 800mg/L.