Determination Of 215 Pesticide Residues In Ginger Using Liquid Chromatography Coupled With Electrospray Ionization Tandem Mass Spectrometry

Pesticides are indispensable material in agricultural production, that used to control or prevention of diseases, insects and weeds. The extensive use of pesticides plays an important role in the increase of food production. However , with the development of science and technology , the species and the number of chemical pesticides are increasing. Because of the long-term large-scale use and abuse of pesticides, the hazards they have brought along with them to food safety and human health have increasingly become the focus of world attention. Ginger is not only condiment and food in our diet , but also one of important export vegetables. During the planting period the ginger is challenged by various plant diseases, insect pests and weeds, affect the yield of the ginger. Pesticides are routinely administered in order to maximise ginger. Together with other types of pesticides in soil, ginger can be contaminated by a variety of pesticides. This situation has let to many countries increased the detection items of pesticide residues in the import/export of ginger, established more and more stringent standards and MRL, which shows a decreasing trend over recent years. It's frequent occurrenced, that China's exports were returned by the importing country . This paper studied extraction, purification and LC-MS/MS detection conditions of pesticide residues in ginger, and establishing the method for determination of 215 pesticide residues in ginger, which cleaning-up by SPE and determined by the high- performance liquid chromatography-tandem mass spectrometry.The main results were as follows:1. We optimized the extract solvent, extract method, clean up method, then established a method, that homogeneous extracted by acetonitrile (containing 1% acetate acid),cleaned-up by Waters Sep-Pak Vac, for extract and clean up pesticide residues in ginger.2. We established the LC-MS/MS detection method for 215 pesticides. 203 pesticides correlation coefficient above 0.99, accounting for 94 percent of the detected pesticides. The limits of detection and the limits of quantification were 0.01~70.45μg/L, 0.04~234.84μg/L respectively.3. The recovery was conducted at two spiked levels which is the LOQ concentration and 4 times of LOQ concentration, the validation results were as follows: the overall recoveries were from 53.6 % to 132.6 % (94.9 % of the recoveries were from 70 % to 120 %), with the relative standard deviations (RSDS) of 0.4 %-25 %.The method is simple. It's sensitivity, accuracy and precision meet the requirements of the multiple pestiside residues. This method is applicable to rapid determination 215 pesticide residues in ginger.