| Gardnerella vaginalis of fox is one of the major pathogens that result in the fox empty pregnant, miscarriages and stillbirths.In 1987, this disease was revealed as zoon as for the first time by ZhongchengYan both at home and abroad. As the microbiological characteristics of Gardnerella vaginalisof fox is different from the human body, Gardnerella vaginalis of fox was named as Gardnerella vaginalis of fox subspecies [7]. Silver black fox, Arctic fox, color fox, red fox and raccoon, and mink are susceptible to infection; Diseased foxes were the main source of infection. Gardnerella vaginalis of fox can spread through mating, and the pregnant mother fox can transmit it to the fetus; It can occur throughout the year. However, infection rates are significantly higher in the breeding period; Different species, different gender and age of fox can be infected. In recent years, epidemics of Fox Gardnerella vaginalis disease are on the rise, which bring serious economic losses to China's fur animals breeding.Four strains of fox Gardnerella vaginalis were isolated and identified from the organs of abortive fox and stillbirth in this topic. The primer was designed by 16s r RNA of Fox Gardnerella vaginalis, 437bp fragment of the nucleic acid was amplified specifically. So the approach has been established that PCR detect Fox Gardnerella vaginalis. It was labeled the PCR product by Digoxigenin, and prepared digoxigenin-labeled nucleic acid probe. Therefore, the diagnostic method was established. It provide a good technical guidance for Fox Gardnerella vaginalis diagnosis and epidemiological surveys. Test1 The Study of Pathogen Isolation on Gardnerella vaginalis of foxThe pathogen of fox, which were from hushan Town of Rizhao in Shandong Province, were isolated and identificated. Susceptibility testing results showed that Gardnerella vaginalis of fox were sensitive to kanamycin and ampicilli nan they were resistance to ceftazidime, and other drugs. This study provided an effect methord for this disease prevention, control and clinical treatment.Test2 The establishment and application of PCR assay for Gardnerella vaginalis of fox A pair of primer was designed from the 16S r RNA gene of Gardnerella vaginalis of fox. 437bp fragment of the nucleic acid was amplified specifically. So the approach has been established that PCR detect Fox Gardnerella vaginalis. It is showed that Fox Gardnerella vaginalis can amplify 437bp specific DNA fragment. While the E. coli, Salmonella, Pseudomonas aeruginosa and Staphylococcus aureus can not.The result of the SENS show that It was detected at least 500 fox Gardnerella vaginalis with the approach. With the approach of the established PCR detecting the 9 suspected strains isolated from different regions of Shandong Province, the results of four stains was positive. Test3 The establishment and application of Digoxigenin-labeled Nucleic Acid Probe for Gardnerella vaginalis of foxThe 16S r RNA gene of Gardnerella vaginalis of fox was shown to be species-specific. A pair of primers were designed for amplifying this 437 bp f ragment in PCR experiments. The PCR products was labeled with digoxigenin as DNA probe for detection of Gardnerella vaginalis of fox. The hybridization assay results showed that the reference strains of Gardnerella vaginalis of fox were positive , no cross-hybridization was detected with DNAs from other bacterial species closely related to Gardnerella vaginalis of fox. The sensitivity result showed that as few as 10×102 of Gardnerella vaginalis of fox could be detected by DIG-labeled probe. The high sensitivity and specificity of the DIG-labeled probe will make it useful in field diagnostic work and epidemic investigation.
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