| Mutant is material which is a gene mutanted or a character can be genetic variation. We can use these materials to separate and identify function of a gene. Brassica napus is one of the most important oil crops in the world, one of the main goals is to clarifing the genetic basis of important agronomic traits controled and cloning genes. Construction mutants library of Brassica napus are rare reports in recent years, it undoubtedly hindered the development of Brassica napus functional genomics. To carry out functional genomics studies of Brassica napus makes large-scale mutant library construction becoming increasingly urgent. Since the maize Ac/Ds transposon system was found, it has been successfully applied in the construction of heterologous plants to separate and identify a gene, for examples, rice,arabidopsis,tomato,tobacco and so on. Utilize the feature that Ds factor can be jump under the effect of AcTapese, we can explore the application of this system to build Brassica napus mutant library, it can provide material to study Brassica napus functional genomics. In the last decade, genetically modified rapeseed and molecular cloning in the significant progress, it improve quality and germplasm resources of Brassica napus. Agrobacterium tumefaciens-mediated genetic transformation is the most common method which is a pure natural biological methods in Brassica napus. It's Simple, low cost, which making it the most widely used methods of genetic transformation.In this study, the plasmid pUbiAc inserted Ac and plasmid pDsBar1300 inserted Ds were transformed into cotyledonpetiole and hypocotyl of Brassica napus cultivar zhongshuang 6 by Agrobacterium tumefaciens-mediated method, After screening, rooting, acclimation, transplanting From the survived 10 Ac transgenic plants,1 positive transgenic individual was identified by PCR assay with a positive frequency of 10%. From the survived 28 Ds transgenic plants,21 positive transgenic individuals were identified by PCR with a positive frequency of 75%. This study will probably supply a new method for the construction of Brassica napus mutant plants and its functional genomics research. |
