Extraction Of Animal Chemicals From The Animal Tissues By Matrix Solid Phase Dispersion

The matrix solid-phase dispersion extraction (MSPD) was applied for exacting sulfonamides in animal tissue samples. The thesis contains two parts:(1) MSPD was applied to the extraction of sulfonamides from liquid samples, such as blood; (2) MSPD was applied to the extraction of sulfonamides from solid samples, such as livers. This is the first time that MSPD is used to prepare blood samples, which will be helpful for the research in the blood in the future.The separation and determination of the analytes were carried out by high-performance liquid chromatography. For the blood samples, the highest recovery was obtained when the diatomaceous earth was used as the dispersion adsorbent and the amount of dispersion adsorbent was 1.0 g, the volume of blood sample was 2.5 ml. The acetone was used as the elution solvent. Under the optimal conditions, the linear range for determining the sulfonamides in blood sample was 0.020-10.0μg/ml, and the average recoveries of three sulfonamides were higher than 87.5%. For the liver samples, the analytes were derivated with fluorescamine and detected with fluorescence detector. The highest recovery was obtained when the diatomaceous earth was used as the dispersion adsorbent and the mass ratio of dispersion adsorbent to sample was 3:1. The acetone was used as the elution solvent. Under the optimal conditions, the linear range for determining the sulfonamides in liver samples was 5.0-1000.0 ng/g. The porcine, chicken and cattle liver samples were analyzed and the average recoveries of seven sulfonamides were higher than 84.6%.