Study On Cold Resistance Of Pistacia Chinensis From Different Provenances

Pistacia chinensis is the main tree species for afforestation in Taihang Mountain Area. Its leaves turn red in the spring and fall, in recent years it has been applied in the garden. In the northern region Pistacia chinensis often suffer from cold temperature, and the study of cold-resistant capability and physiological indicators had not been reported yet. So it is significant to determine the hardiness of Pistacia chinensis which is important in appraising, popularizing and utilizing the cold resistance Pistacia chinensis. In this paper, Pistacia chinensis was studied by main component analysis, variance analysis and subordinate function values analysis. The cold resistance characteristics of Pistacia chinensis were systematically investigated from the levels of physiology and biochemistry, based on cold resistance system, aims to study the cold resistance characteristics and mechanism. The major results were summarized as follows:(1) The changes of physiological and biochemical indicators on the spring twigs and summer twigs were basically same during the overwintering. The changing range about transpiration rate, water saturation deficit, relative water content and SOD activity of summer twigs was larger than those of spring twigs. The ion leakage percentage and MDA in branches of summer twigs were higher than those of spring twigs. The content of soluble sugar and soluble protein of summer twigs were lower than those of spring twigs. The results showed that we should take protective measures for Pistacia chinensis before December.(2)It showed that the activities of SOD and POD of one-year and two-year Pistacia chinensis twigs increased early and decreased later, the content of MDA,soluble protein and soluble sugar increased with the temperature decreasing under freezing resistances. The relative electric conductivity increased as a S-curves with the temperature decreasing. According to the Logistic equation, the semi-lethal temperature (LT50) for twigs of one-year and two-year Pistacia chinensis twigs were -24.9℃and -26.6℃, respectively.(3) It proved that the relationship between ion leakage percentage, SOD activity, POD activity, water saturation deficit and relative water content were closer during the overwintering; The relationship between ion leakage percentage, the content of MDA,the content of soluble protein and soluble sugar and the treatment temperature were closer with the temperature decreasing. (4)It showed that the transpiration rate of different provenances Pistacia chinensis twigs increased with the temperature decreasing under freezing resistances; The activities of SOD and POD of different provenances Pistacia chinensis twigs increased early and decreased later; The content of MDA,soluble protein and soluble sugar of different provenances Pistacia chinensis twigs increased with the temperature decreased; The relative electric conductivity increased as a S-curves with the temperature decreased.(5) According to the Logistic equation, the semi-lethal temperature of different Pistacia chinensis provenances were obtained, it showed that the cold-tolerance of different Pistacia chinensis provenances could be arranged from strong to weak as Mazhuang Town>Linqi Town>Hejin Town>Xida Town>Yuankang Town>Dongyao Town>Tuonan Town>Donggang Town>Guxin Town>Guanfang Town.(6)According to subjection function value analysis,it showed that the cold-tolerance of different Pistacia chinensis provenances could be arranged from strong to weak as Mazhuang Town>Linqi Town>Hejin Town>Xida Town>Yuankang Town>Dongyao Town>Tuonan Town>Donggang Town>Guxin Town>Guanfang Town.(7)It showed that these two results are consistent. According to the Logistic equation to obtain LT50 can serve as a rapid determination of Pistacia chinensis simple method of cold resistance.