Effect Of Copper In Pig Feees On Content Of Copper And Physiological Characteristics Of Brassica Juncea

The purpose of this study was to investigate the effects of on seedling growth and physiological characteristics of Brassica juncea under different concentration copper of pig feces. An experiment is that Brassica juncea was grown in pots added with 3kg soil, three different amounts of pig feces (150g,300g,450g) were added into each treatment. It was conducted to study the effects of different levels copper in pig feees on Cu accumulation, the physiological activities of antioxidative enzymes and the content of antioxidants, the content of gibberellic acid and N6-benzyladenine in Brassica juncea.1.At 60 days after cultivation, Cu content in Brassica juncea tissue were directly correlated to the amounts of Cu applied into the soil. With a single application of 450g manure /pot, soil Cu was found to be 147.03 mg Cu/kg, which exceeded the national soil quality standard of 100 mg/kg for Cu. Experimental manure applieations at 150 and 300g/Pot increased soil Cu contents, but did not raise soil Cu levels beyond the national soil quality standard of 100mg/kg.2.Copper accumulation coefficient of root biomass was increased than that of aboveground biomass for eaeh Cu application rate examined. Copper concentrations in the roots were linearly related to soil Cu, which may be deseribed by this linear equation:Y=0.3011X+3.821, R2=0.8715.3. At 60 days after cultivation, total fresh Brassica juncea biomass decreased as Cu application rates increased(p<0.05). Results seemed to suggest that plant roots and aboveground biomass exhibited different Cu toxieity tolerance threshold levels. Significant aboveground biomass reduction occurred at the 300mg/pot manure application rate;where as root biomass reduction was observed at150mg/pot manure applieation rate. Results appeared tosuggest that the roots were more sensitive to Cu toxicity;root inhibition preceded aboveground biomass reduction.4. From 30 day after cultivation, SOD and CAT activities increased significantly at the 150 and 300mg/pot manure application rate than 450mg/pot manure application rat.At 45--60 days after cultivation, SOD and CAT activities decreased significantly at the 450mg/pot manure application rate. From 15--45 day after cultivation, GR, GPX and GST activities of leavies increased significantly as Cu application rates increased (p<0.05), which may have resulted in a decrease in stress resistance of Brassica juncea at 60 days after cultivation.5.The content of MDA and H2O2 in Brassica juncea leavies increased significantly as Cu application rates increased (p<0.05).Especially, the content of MDA and H2O2 in Brassica juncea leavies rapid increaseing at the 300mg/pot manure application rate.6. At 60 days after cultivation,use the RP-HPLC method to analyze the content of GSH in Brassica juncea leavies and roots. The content of GSH in Brassica juncea leavies and roots increased significantly as Cu application rates increased at the 300mg/pot manure application rate, which may have resulted in a decrease in stress resistance of Brassica juncea at the 450mg/pot manure application rate.The content of GSH in Brassica juncea roots increased extreme significantly than leavies at the 300mg/pot and 450mg/pot manure application rate.7. After optimized hormone extraction conditions and chromatography conditions, the optimal chromatographic conditions were selected, which included the mobile phase were composed of methanol and 0.075% acetic acid (45:55, V/V),the flow rate was 0.7 mL/min, the column temperature was 30℃and the wavelength of detection was 254nm, respectively. The methods of the two hormones isolation were established in Brassica juncea. The results showed that the contents of GA3 and 6-BA in shoot and root of Brassica juncea were heightened with the increasing concentration of copper treatments, the contents of GA3 and 6-BA in shoot of Brassica juncea is higher than root of Brassica juncea.The pollution of high level copper in pig feces caused Cu accumulation in soil, lead to exceed standard and Cu accumulation in plant, which affect the physiological activities of antioxidative enzymes,antioxidants, the content of gibberellic acid and N6-benzyladenine in Brassica juncea. The best way of controlling pollution is to estbalish relevant limiting standard,to limit the Cu addition in the pig feed and to fertilize manure reasonably according to the types of soil.