| This thesis was designed to study the effect of dietary supplementation of methionine on ruminal digestion, metabolism, and blood parameters in goats, to supply technology supporting for nutritional and physiological regulation of limiting amino acids in ruminants.A trial with three ruminanlly cannulated catheter goats (initial body weight:20±2.5 kg) were allocated to three dietary treatments in a 3 X 3 Latin square design to estimate the effects of dietary methionine levels on ruminal digestion and metabolism, blood parameters in goats. Goats were fed the total mixed diets containing three levels of methionine (0.10%,0.20% and 0.30%). The results showed:the dietary methionine levels did not affect the ruminal pH value (P>0.05); the average ruminal concentration of NH3-N was 11.79,8.66,9.32 mg/dl, respectively (P>0.05); there was an decreasing tendency for the ruminal pH value and NH3-N concentration with the increasing of dietary methionine level (P>0.05); there were no significant difference (P>0.05) for the ruminal concentration of total volatile fatty acids, acetic acid, propionate acid, butyrate acid, valerate acid and the ratio of acetic acid to propionate acid (P>0.05); the dietary methionine did not affect the average blood concentration of TP, AMY, LDH, BUN and INS (P>0.05); the average content of Glucagon for 0.20% methionine treatment was significantly higher (P<0.05) for the 0.10% and 0.30% methionine treatment, and that for the 0.30% methionine treatment was significantly higher (P<0.05) than that for the 0.10% methionine treatment. It is concluded that dietary supplementation of methionine is beneficial to the ruminal digestion and metabolism in goats, and the best supplemented level is 0.20% in this study.
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