Preliminary Study About Molecular Mechanism Of Inflammatory Bowel Disease Of Fish

Inflammatory bowel disease (IBD) is a chronic inflammatory disease and autoimmune disease, however, the pathogenesis is still unclear. Scientists generally agreed that the environmental factors, genetic factors and abnormal immune responses are all relevant to IBD. Research shows that one of the possible mechanisms of IBD is the expression of Peptide transporter 1(PepT1) in colonic epithelial cells under abnormal conditions, which transport some bacterial products, such as N-formylmethionyl-leucyl-phenylalanine (fMLP) into the intestinal epithelial cells induce inflammatory response. PepTl is an oligopeptide transporter transport di-and tripeptides and some structurally similar drugs. PepTl transport fMLP into intestinal epithelial cells induce inflammation has been found in Caco2-BBE cells. It can bind with formyl peptide receptor(FPR) to stimulate NF-κB and AP-1 activities, which may lead to the activation of inflammatory responses and mucosal damages.In our experiments we study the relationship between interleukin 16(IL-16) and inflammatory bowel disease. We use a model fish Tetraodon nigroviridis to construct a fish intestinal inflammatory model through the injection of IL-16. We systematically studied the role of IL-16, the expression and transport activity of PepTl in intestinal inflammation. Our results provide the first evidence about IL-16 induce intestinal inflammation through up-regulating PepTl. The results not only provide new ideas about the prevention of fish immune diseases, but also provide potential new research models and therapeutic targets of clinical treatment research and drug development about human IBD.