Screening Of Fungi For Efficient Degradation Of Cellulose In Oil-tea-cake

This article simplely described the use of Camellia's various products ,and how to produce protein feedstuff from oil-tea-cake by the solid state fermentation technology. Because oil-tea-cake contains a large number of cellulose which can not be digested by domesticated animal ,and owns tea saponin maybe poison livestock when it largely present.So the strains fermented tea cake should not only have the ability to degrade cellulose in tea, but also own the ability to degrade a large number of tea saponin.This study from the soil, garbage and rotting leaves through such as Congo red medium, carboxymethyl cellulose medium and so on mediums screened out of L-1, L-3, L-4, L-5, L-6, L-2 6 strains. Then used those six strains to individually ferment the medium which was composed of Camellia cake as the main nutritional components.By comparing different strains of exo cellulase activity (C1), endo-cellulase (CMCase), filter paper activity (FPA) and the the ability to degrade tea saponin, had known that the strain L-3 was the best strain to decompose the cellulose of Camellia cake,at the same time , the strains L-1, L-4 were the excellent strain. After screening the best and excellent strains, comparing the three types of activity between single fermentation and mixed fermentation foud that L-1+L-3 mixed fermentation was better that single fermentation.All of those proved the ability of L-1+L-3 mixed fungi about degradating oil-tea-cake's cellulose meal was better than the ability of a single strain. Then by comparing all three enzymes of L-1 +L-3, L-1 + L-4, L-3+ L-4, L-1 + L-3+ L-4, determined that the best mix combination was L-1+L-3.