Development Of Porcine Parvovirus-Japanese Encephalitis Bivalent Inactivated Vaccine And Porcine Parvovirus-Pseudorabies Virus Bivalent Inactivated Vaccine

Porcine parvovirus, pseudorabies virus and Japanese encephalitis viruses are the main cause of reproductive failure in sows pathogen, in order to prevent these three diseases, the study first use of the laboratory isolation and identification of porcine parvovirus WH-1 strain and pseudorabies E A toxic strain was developed for simultaneous control porcine pseudorabies virus and the second oil emulsion inactivated vaccine, but also provided the use of Hubei Academy of Agricultural Sciences WH-1 Japanese encephalitis virus strains of porcine parvovirus and prepared Second Japanese encephalitis virus vaccine inactivated oil emulsion. Results were as follows:1.Develop Porcine parvovirus-pseudorabies virus in Oil Emulsion Inactivated VaccineConcentrated using dialysis bags prepared high titer porcine pseudorabies virus fluid and venom as a system with a vaccine antigen, inactivated formaldehyde liquid to collect the virus, two types of inactivated virus,such as volume of liquid mixture, add oil emulsion emulsified made of porcine parvovirus-combined inactivated pseudorabies virus vaccine. Following physical properties of the vaccine, after testing and sterility testing was carried out safety tests and immunity tests, the results confirmed that the vaccine on laboratory animals and the animals are safe and reliable, immune pigs resulting from the corresponding two kinds of antibodies, antibody levels and single seedlings rather.2. Porcine parvovirus-Japanese encephalitis virus in Oil Emulsion Inactivated VaccineConcentrated using dialysis bags prepared high titer PPV solution, Hubei Academy of Agricultural Sciences provides high titer of Japanese encephalitis virus solution, two types of inactivated virus fluid volume ratio of 3:1 and 1:1 by two mixed ratio mode, and then got two kinds of emulsion system PPV-Second Japanese encephalitis virus oil emulsion inactivated vaccine. Following physical properties of the vaccine, after testing and sterility testing, carried out safety tests and immunological tests, the results show that the vaccine on laboratory animals and the animals are safe, reliable, immune pigs could induce high level of antibody, Parvovirus HI antibody most up to 1:10240, JE ELISA antibody up to 1:40960. PPV-Japanese encephalitis virus in Oil Emulsion Vaccine pig antibodies as a whole and each single vaccine induced significant antibody levels.This study porcine pseudorabies virus and the immune provides the possibility of joint, but also for porcine parvovirus disease and epidemic encephalitis B provides the possibility of joint, and the experimental study confirmed the preparation of the combined vaccine can be induced after immunization, and one pig the same antibody titer vaccine, but also to simplify immunization procedures and reduce the stress brought about immunization, can achieve a pin against the purpose of sick, development to meet the scale Pig's immediate needs.