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The Resistance Of Cucumber Induced By Exogenous Salicylicacid And Methyl Jasmonate Respectively To Frankliniella Occidentalis

Posted on:2010-01-31Degree:MasterType:Thesis
Country:ChinaCandidate:F YangFull Text:PDF
GTID:2143330302955270Subject:Insect ecology
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With the world-wide quarantine Western flower thrips (Frankliniella occidentalis) the successful invasion of our country, its crops and cash crops in China caused serious economic losses and the potential threat is causing great concern of more and more widely. In order to better prevention and control of the invasion biology, a common cash crop in this article cucumber cucumber (Sativus Linn) as a host plant, using ecological and physiological and biochemical tests, such as a combination of research methods, comparative study of induction of different exogenous Methyl Jasmonate factor (Methyl jasmonate, MeJA) and salicylic acid (Salicylicacid, SA) and other treatment directly host resistance of cucumber to the survival of F. occidentalis fitness development and indirect effects of insect resistance to western flower thistle the impact of selective feeding horses, and from the body after the induction of cucumber volatile material change and to explore or resolve the differences between exogenous cucumber-induced mechanism of insect resistance, resistance to the use of plants for the prevention and control of the perspective of F.occidentalis provide a theoretical basis. The main results or conclusions were as follows:(1)Salicylic acid, methyl jasmonate-induced cucumber on the survival of F.occidentalis fitness effects of.Comparison of different concentrations (5mmol.mL-1, 0.5mmol.mL-1, 0.05mmol.mL-1) SA, different concentrations (1mmol.mL-1, 0.1mmol.mL-1, 0.01mmol.mL-1) MeJA, insect injury (Damage by thrips' feeding, DTF), mechanical damage (Mechanical damage, MD) and control (healthy plant) (Control, CK) 9, under different host plants of cucumber on the survival of F. occidentalis fitness degree of influence compared to the control results show that CK, SA, MeJA-induced cucumber processing can effectively produce insect-resistant, and significant extension of the F.occidentalis development time, pupal weight suppression, reducing the survival of F.occidentalis fitness; MD CK have to deal with compared to the control of insect resistance, for the F. occidentalis of growth and development are not significantly affected, but pupal weight increased, so that MD can deal with some of the cucumber to enhance the survival fitness of insects; but DTF compared to the control CK, induced by the best of insect resistance, inhibit the growth and development of F. occidentalis most obvious; MeJA to deal with compared to SA treatment, average length of nymph development time Id, pupal weight reduction of more than 0.01mg on MeJA-induced plants is more effective to improve insect resistance; inducible factor for several other western flower thrips than the obvious impact.(2) Salicylic acid, methyl jasmonate-induced resistance of cucumber produced some of the material basis of in vivo.Comparison of the different concentrations of SA, MeJA, DTF, MD and CK9 under different host plants to deal with the physical body type cucumber nutrients (protein, amino acids, free fatty acids, fructose, water content, chlorophyll), secondary substances (tannins, flavonoids) and defense-related enzymes [(Px (Peroxidae, POD), polyphenol oxidase (Polyphenol oxidase, PPO), phenylalanine ammonia-lyase (Phenylalanine ammonia-lyase, PAL), protease inhibitor (Proteinase inhibitor, PI)] of different dynamic analysis of their role in plant resistance, insect resistance results show that the plants in these three areas by the combined effect of the material. which physiological nutrient substances lower the overall content is not conducive to F.occidentalis growth and development, not by a particular decision and deal with MeJA and SA were lower compared to the control of the CK type of plant nutrient content of the material; secondary substances, the flavonoids and tannin content compared to the resistance of host plants closer relationship between the correlation coefficient 0.67, compared with the control CK and SA, MeJA processing can significantly increase the flavonoid and tannin content; defense enzyme, PPO and PI activity relative to the resistance of cucumber is more important, while the POD and insect-resistant effect of PAL enzyme activity was not obvious, which can be induced by SA main POD, PAL enzyme activity to increase, MeJA was mainly to improve the PPO, PI. therefore the primary role of SA resistant material may be based on host plant cucumber by physiological substances in the body decline caused by a leading role; MeJA and insect resistance may be the material basis there was no significant difference between the three.(3) Salicylic acid, methyl jasmonate-induced cucumber F.occidentalis on the impact of selective feeding.F.occidentalis compared to salicylic acid (0.5mmol.mL-1), methyl jasmonate (0.1mmol.mL-1), insect injury, mechanical damage and cleaning the control to deal with five kinds of different types of host plants from the cucumber eat more, as well as the different host plant differences in volatile compounds. The results show that the state of hunger followed by the F.occidentalis insect injury, methyl jasmonate and salicylic acid to deal with increasing selectivity, the performance of seedlings of the most clean hobby; the other five kinds of host plants to deal with the volatile material content of cucumber and group Most of the insects into a wound treatment, and then followed by methyl jasmonate, salicylic acid, mechanical damage and cleaning the control seedlings, which were clean and mechanical damage seedlings volatile component content of more than 85% of ingredients are green, insects Methyl Jasmonate to deal with injuries and more than 75% volatile terpenoids are mainly (E)-4,8-dimethyl-1,3,7-Azelaic triene, D-penten-2, Class terpene, farnesene, ocimene, etc., which may exist with the approach-avoidance nature of the material.
Keywords/Search Tags:cucumber, western flower thrips, secondary metabolites, enzymes, volatile, development
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