Researches On Tissue Culture Of Fan Flower Scaevola Nitida R.Br

Australia fan flower Scaevola nitida R.Br is a good flowering shrubs belong to Goodeniaceae, Scaevola L. Tissue culture technology was systematically researched in this paper. Using leaf, petiole, stem and stem nodes as explants, regeneration systems via direct organogenesis and indirect organogenesis were established based on researches of the influence of explant types, plant growth regulators and culture conditions. Main results were as following:(1) Two-step surface sterilizing was used to leaf explants. Leaf explants were first treated with 75% alcohol for 30 seconds and then treated with NaClO and HgCl2 of different concentration for different times. The results showed that the best treatment was to sterilize the explants with 0.1% HgCl2 for 10 min.(2) For direct organogenesis, concentration of 6-BA and NAA had extremely significant effect on shoot induction ratio, average shoot number and average shoots height, while that of the interaction of the two factors were not significant. MS+1.5 mg/L 6-BA was the suitable media for direct shoots induction from leaves.(3) Effects of pH,6-BA,2,4-D and explant types to callus induction were analyzed. Results showed, good callus induction could be obtained by culturing petiole explants in the media MS+0.5 mg/L 6-BA+1.5 mg/L 2,4-D, pH 6.4, which callus induction ratio reached 92.22% and the callus were yellowish green.(4) For callus differentiation, good results of differentiation rate 64.45% and an average shoot number 1.26 could be obtained with MS+0.5 mg/L 6-BA+0.05 mg/L NAA.(5) Concentrations of 6-BA and NAA and their interaction had significant effect on axillary bud proliferation. The proper medium was MS+1.0 mg/L 6-BA+0.5 mg/L NAA.(6) The concentration of sucrose and organic nutrients had extremely significant effect of shoots growth. MS with 3% sucrose and 15 ml/L organic nutrients stock (100×) could make the plant stronger.(7) Experiments on the effect of NAA to rooting showed that good rooting results could be obtained with 1/2MS +3% sucrose+0.1 mg/L 6-BA+0.3 mg/L NAA and replacing agar with perlite, which rooting percentage was 92.22%, average root number was 6.02, average root length was 3.88cm and the roots were stronger.(8) Orthogonal experiments on the effect of the concentration of sucrose, macro elements, NAA and IBA to rooting were conducted. Results showed that the concentration of sucrose, macro elements and NAA had significant effect on average root number and average root length; using 1/4MS + 3.5% sucrose + 0.5 mg/L NAA+1.0 mg/L IBA or 1/4MS + 3% sucrose + 0.2 mg/L NAA+1.5 mg/L IBA as media, rooting ratio could reach 92.22% and 91.11%, average root number were 3.01 and 2.83, average root length were 2.62 cm and 2.10 cm in respective.Using leaf as explants, through direct organogenesis is the best way to get a large quantity of transplanting ginseng.