Comparative Study On Flavonoids In Different Treatments

Ginkgo (Ginkgo Biloba L.) for the genus GinkgoGinkgo biloba, also known as white-fruit trees, such as maidenhair tree. Ginkgo flavonoids are the main active ingredient in one of the coronary blood vessels have the expansion, to inhibit lipid peroxidation, blood fat, increase cerebral blood flow, such as the role of the clinical use of cardiovascular, cerebrovascular, atherosclerosis and other diseases. This experiment on the fresh leaf of Ginkgo flavonoids in the analytical methods, extraction and purification technology, fingerprint studied.Main research contents are as follows:1. Determination of the the content of flavonoids in pratense Ginkgo biloba and extracts.To the total flavonoid glycosides of Ginkgo biloba as an index component, using HPLC assay methods, Liuyang, Hunan fresh Ginkgo biloba leaves 5-year total flavonol glycoside content of the dynamic changes with the seasons; compared three different places (Liuyang, Hunan, Jiangsu Pizhou, Yunnan Tengchong), as well as medicinal herbs in fresh, dry, dry, dry state of the total flavonol glycosides differences. The results showed that:the beginning of period (5,6 month) the total ginkgo flavone glycosides content of the highest; three different places of the total content of flavonol glycosides no significant difference; leaf flavonoid content higher than the dry goods, fresh herbs in the process of drying some flavonoids loss, the loss rate of about 28%, which will fresh herbs ginkgo biloba resources provide the basis for the use of depth.2. Fresh leaf of Ginkgo flavonoids extraction technology researchOrthogonal test of flavonoids in Ginkgo biloba leaves fresh extraction and purification process optimization, set the first homogenate fresh Ginkgo biloba leaves and then water and purified by macroporous resin processing parameters, the best prepared extracts of total flavonoids in more than 30%, process is stable and easy, suitable for industrial production.3. Establishment of HPLC fingerprint of extracts of Ginkgo fresh, dried biloba.The establishment of a fresh, dry extract of Ginkgo biloba fingerprint of HPLC, the use of similarity as a fingerprint evaluation parameters, the different origin of Ginkgo biloba extract, as well as between the HPLC fingerprints of Ginkgo biloba and the reference substance of the similarity between the fingerprint. The results showed that:fresh, dried ginkgo biloba extract ginkgo biloba extract with the reference substance reached the similarity of more than 0.90; through cluster analysis of standardized data can be fresh, dried ginkgo biloba extract clear distinction into two types.