| Purposes:through on diabetes erectile dysfunction function obstacles (DMED) patients of TCM differentiation card points type for survey and Statistics analysis, research DMED of card type distribution law, while joint discussion chain urea junior bacteria pigment (STZ) celiac injection method established diabetes erectile dysfunction function obstacles animal model and PTN gene turned dye big rat of fat organization sources stem cell (ADSCs) to get for gene treatment of seed cell of feasibility and the by PTN gene modified hou of ADSCs treatment DMED big rat of effectiveness.Methods:DMED in accordance with the international index of erectile function in patients with-5 (IEF-5) score and TCM syndrome typing table according to Qi, Yin and Yang-deficiency and blood stasis and phlegm-dampness syndromes classified. The ADSCs also isolated and cultured in vitro in rats using morphology and flow cytometry to identify, PTN gene transfection ADSCs antibiotic G418 filters the transfected cells; qPCR, Western blot on the filtered cells was detected. And effect of Streptozotocin diabetic rats and rat model of morphine to filter out DMED Park. Animals were divided into four groups:a control group; B, saline control group; C, fat stem cells in the treatment group:d, with PTN gene of adipose-derived stem cells in the treatment group. Rats after 7 days of treatment with intracavernous pressure (ICP) and an average of carotid arterial blood pressure (MAP) determination.Results:Yang-deficiency and blood-stasis syndrome score was negatively correlated with DEF-5 score, mild ED syndrome of syndrome of deficiency of both Qi and yin and Yang deficiency and blood stasis; moderate ED syndrome for Yin deficiency of syndrome of deficiency of both Qi and yin and blood stasis; severe common ED syndrome of Yang-deficiency and blood-stasis syndrome of Yin deficiency and blood-stasis syndrome. While DMED successful model in rats managed to get high with PTN gene expression of adipose-derived stem cells, Western blot tests showed stable expression of PTN protein. After the treatment, erectile dysfunction with PTN gene of adipose-derived stem cells in the treatment group improved significantly, the difference was statistically significant.Conclusion:DMED-Yang deficiency and blood stasis in patients with erectile dysfunction is poor, tip DMED pathogenesis may blood stasis as the core and Foundation. Intraperitoneal injection of STZ-induced diabetic rats by appropriate doses for 50mg/kg, after STZ injection showed typical symptoms of diabetes, blood sugar significantly increased impaired erectile function, screening can be effectively screened ED APO model. Experiments for stable expression of PTN adipose tissue-derived stem cells and laid the Foundation for the treatment of diabetic erectile dysfunction through PTN ADSCs treatment, DMED in rats with damaged erectile dysfunction can be a degree of recovery. |
