Effect Of Postoperative Intestinal Obstruction On Nesfatin - 1 Expression In Hypothalamus And Brainstem Of Rats

Objective:To investigate the effects of abdominal surgery-induced postoperative ileus on nesfatin-1-immunoreactive (ir) neurons expression in the rat brain.Methods:Rats were anesthetized with sulfuric acid butabarbital and abdominal surgery was performed. Two hours after abdominal surgery, rats were transcardially perfused. The brain tissues were processed for double immunohistochemical labeling of Fos and nesfatin-1.Results:There was an obvious increase of c-Fos expression in neurons of the supraoptic nucleus (SON)(t=6.9, p<0.01), paraventricular nucleus(PVN)(mpPVN (t=17.2; p<0.01), mmPVN (t=25.8, p<0.01),1mPVN (t=19.5; p<0.01), apPVN (t=22.1; p<0.01)), locus coeruleus (LC)(t=2.6, p<0.05), Edinger-Westphal nucleus (EW)(t=10.74, p<0.01), rostral raphe pallidus (rRPa)(t=2.57, p<0.05), nucleus of the solitary tract (NTS)(t=10.46, p<0.01) and ventrolateral medulla (VLM)(t=7.86, p<0.01), but nesfatin-1-immunoreactive cells did not differ(P>0.05). The statistical analysis showed that among the activated c-Fos neurons,99%in the SON,71%in the anterior parvicellular PVN,47%in the lateral magnocellular PVN,41%in the medial magnocellular PVN and9%in the medial parvicellular PVN could express nesfatin-1; in the brain stem, after abdominal surgery, the activated c-Fos neurons91%in the LC,82%in the rRPa,74%in the EW and VLM,14%in the NTS could express nesfatin-1.Conclusion:Abdominal surgery can activate the nesfatin-1-ir neurons expressed in some feeding-related nuclei in the hypothalamus and brain stem, suggesting a possible implication in the control of food intake and gastric emptying disorder.