Study On The Protective Effect Of Cordyceps Sinensis On Ischemic Apoplexy

Ischemic stroke is higher in today’s society incidence of disease, higher mortality and morbidity, more abrupt onset and complex of pathological mechanisms, difficult to receive the desired effect of conventional therapy, traditional Chinese medicine make an important rote in the prevention and treatment of cerebrovascular disease. The use of Chinese medicine has opened up a new direction for the treatment of cerebrovascular disease.Cordyceps is a traditional specialty of precious Chinese medicine, because of its medicinal value and low toxicity,it gradually gets attention of scholars at home and abroad. Cordyceps has a large number of pharmacological effects,such as anti-inflammatory, anti-tumor, anti-anoxia,lowering blood sugar. Early experimental study of the research group found that weight-bearing mouse model of Cordyceps sinensis in cold water can prolong gasping time, thereby enhancing its ability to survive, which may because of reducing the lactic acid content and regulating biochemical indicators. Cordyceps in rat MCAO model can significantly improve the neurological symptoms in rats after ischemic brain injury, reducing infarct and cerebral edema, with a significant neuroprotective effect; the mechanisms of cerebral ischemic symptoms protection may be inhibit the expression of inflammatory cytokines such as TNF-α, IL-1β, ICAM-1, VCAM-1,reducing its levels in the brain, and thus play a protective role in brain. In this study, we use rats with middle cerebral artery occlusion model to confirm the neuroprotective effect of Cordyceps sinensis, and establish the foundation of further exploration for neuroprotective effects of Cordyceps sinensis by selecting and testing functional protein.Objective:Previous studies have confirmed that Cordyceps has significant neuroprotective effects in cerebral ischemia, for further study of its mechanism, we make use of protein chip technology to explore the therapeutic mechanism of ischemic stroke.1. To investigate the neuroprotective effect of Cordyceps in ischemic stroke.2. To select functional proteins for neuroprotective effect of Cordyceps sinensis.3. To test functional proteins for neuroprotective effect of Cordyceps sinensis.Methods:1. Using enzyme-linked immunosorbent assay (ELISA method) to explore the transformation of SOD, GSH-Px and CAT after cerebral ischemia and the influence of Cordyceps.2. Using neurological deficit score to study the effect of Cordyceps sinensis on neurological evaluation of cerebral ischemia3. Using protein chip technology to study the proteins of neuroprotective effect of Cordyceps sinensis on focal cerebral ischemia.4. Using Western blot hybridization technique (Western blot) to verify results of protein chip.Results:1. Use ELISA method to explore the transformation of SOD, GSH-Px and CAT after cerebral ischemia and under influence of Cordyceps, the results show that levels of SOD, CAT, GSH-Px were prominently increased in Cordyceps group (P<0.01), Cordyceps make a protective role in the brain by reducing the toxicity of free radicals after cerebral ischemia.2. Compared with the model group,72h ischemic neurological deficit scores were significantly lower in Cordyceps group (P<0.01), Cordyceps have neuroprotective effects in ischemic stroke.3. The result of protein chip showed that the expression of Granzyme B、TGF-β2、 PDGF-B、Ras、MMP-16、Bcl10、TNF-R2、VEGFR2、Fas-ligand、Integrin-β5、 TMP-1、ER-β、IGF-1、VEGFR1、TACE、IPO-38 Proliferation Marker、Caspase 8 were lower and NF-κB、MMP-2、GCS、TGF-β3 were higher in rat MCAO model, Cordyceps can improve the expression of PDGF-B (P<0.05)、TGF- β2 (P<0.05)、 TNF-R2 (P<0.05)、VEGFR1 (P<0.05)、VEGFR2、Fas-ligand、NF-κB、MMP-2、 GCS、TGF-β3、Granzyme B、Ras、MMP-16、Bcl10、Integrin-β5、TIMP-1、 ER-β、IGF-1、TACE、IPO-38 Proliferation Marker、Caspase 8 in rat brain tissue after cerebral ischemic, these functional proteins involved in a variety of process of apoptosis, angiogenesis, inflammation, etc.4. Compared with sham group, expression of TNF-R2, VEGFR1 in model group are up-regulated, and there is a significant difference (P<0.01 or P<0.05); compared with the model group, expression of VEGFR1 increased, expression of TNF-R2 (P<0.01) decreased in cordyceps group.Conclusion:1. Cordyceps can significantly increase levels of SOD, GSH-Px,CAT and improve symptoms of ischemic brain nerve defects, have neuroprotective effects.2. The mechanism of Cordyceps in improving ischemic brain symptoms may be increase the expression of VEGFR1, reduced the expression of TNF-R2, through comprehensive coordination of apoptosis, angiogenesis, nerve regeneration and inflammation, and other physiological and pathological processes, play a neuroprotective effect.