Effect Of Wuzi Yanzong Pill On The Expression Of Cox7a2 And Claudin - 11 In Rats With Kidney Deficiency Deficiency Syndrome

Recently,the number of male patients suffering from infertility shows the trend of incidence on the rise in the world. The relevant research indicates that the infertile couples can account for 15% of the married couples,which is alone caused by man’s reason up to 20% and caused by combined factors accounted for 40%-50%. According to the Traditional Chinese Medicine,the pathogenesis of male infertility is recognized by the majority as deficiency of the kidney,accompanied with stagnation of the liver-Qi, phlegm-dampness,blood deficiency and blood-stasis. The traditional Chinese therapy for male infertility is based on different syndrome.The treatments are chiefly warming the kidney yang,nourishing the kidney yin and replenishing the kidney essence. Wuziyanzong pill (WYP), a classical formula in Chinese Medicine is widely utilized for the treatment of male infertility with kidney essence deficiency. But the overall pharmacodynamic mechanism hasn’t been fully understood,therefore,effective research ideas and approaches are needed to investigate the mechanisms.This thesis is divided into two sections-literature review and experimental study.Literature review systematically summarizes the current research on sterility from the perspective of TCM(Traditional Chinese Medicine) in. recent 3 years. It discusses the overview of Chinese medicine treating male infertility, the therapy ways and the relevant formulas. It also reviewes the clinical and experimental research of WYP in recent years.The relationship between Cox7a2、ERK、Claudin-11 with spermatogenesis function from the perspective of modern medicine is studied.which paved way for our further research.This experimental study contains four parts.OBJECTIVE:To observe the effects of WYP on Cox7a2 gene, Claudin-11 expression and to investigate extracellular signal-regulated kinase (ERK) phosphorylation in rat with kidney essence deficiency syndrome.METHODS:Five groups of Sprague-Dawley male rats (5 in each group) were given vehicle, Tripterygium Wilfordii glycosides(GTW) alone (20mg/kg), or GTW followed by WYP (0.5,1.0, or 2.0 g/kg daily) respectively for 30 days. Cox7a2 mRNA expression in testis was determined by real-time reverse transcriptase polymerase chain reaction. The extracellular signal-regulated kinase1/2 (ERK1/2) phosphorylation level and Claudin-11 expression were assessed by western blot analysis.RESULTS:GTW induced increased cox7a2 mRNA expression and an activation of ERK as well as a decline of Claudin-11 expression. Compared with GTW group, ERK phosphorylation level in high-dose WYP group decreased greatly with upregulated Claudin-11 expression. Furthermore, high-dose and mid-dose WYP downregulated cox7a2 mRNA levels.CONCLUSIONS:WYP protected the impaired spermatogenesis possibly through mediating mitochondrial energy metabolism and blood testis barrier function via ERK pathway.