Study On SERS Spectra Of Six Kinds Of Intestinal Pathogens

Surface Enhanced Raman Scattering (SERS) technology is a testing method ofsubstances which has many advantages, such as highly sensitivity, little interferencefrom water and quenches the fluorescence ea al. So, its applications has beenresearched widely since it was discovered. In the study of Applications insurface-enhanced Raman scattering spectroscopy, much attention has been paid todetection and analysis of biological molecules by SERS technology. In this paper,we discuss detection and analysis of different types of bacteria by SERS technology.Colloidal Ag nanoparticles which is prepared by microwave method is applied todetect six types of intestinal pathogenic bacteria. The UV-vis absorption spectra ofColloidal Ag nanoparticles mixed with six kinds of bacteria before and after weredetermined by TU-1900ultraviolet-visible spectrophotometer, the adsorption ofColloidal Ag nanoparticles and bacteria after mixed could be deduced. Meanwhile,the SERS effect of six kinds of intestinal pathogenic bacteria on colloidal Agnanoparticles were studied with the Portable Raman spectrometer(R-3000). SERSeffect is significantly, and the SERS spectra of staphylococcus aureus, proteus,Escherichia coli,Salmonella enteritidis, Salmonella paratyphoid B and Salmonellatyphoid was obtained.The article first reported the study on SERS spectra ofstaphylococcus aureus and proteus. Not only the position of Raman vibration peaksbut also intensity of the six bacteria is obviously different,so SERS can be used foridentification and distinction of E. coli, Staphylococcus aureus,Proteus,Proteus;Salmonella enteritidis, Salmonella paratyphoid B and Salmonella typhoid,andidentified frequency and vibration of Raman spectra of six kinds of bacteria.