In Vitro Immunofluorescence Imaging Of Gastric Cancer Cell Line MGC80-3 Based On Cadmium Telluride Quantum Dot Probe

Objective:In this experiment, tumor-specific quantum dots (QDs) which could bind gastric tumor cell line MGC80-3specifically was employed for immunofluorescence.Methods:Primary QDs were grafted onto the tumor-associated glycoprotein72(TAG-72) monoclonal antibody CC49to produce CC49-QDs that could specifically label tumor cells. Following a series of tests on the diameter and emission spectrum of CC49-QDs, they were employed in immunofluorescence analysis.Result:Transmission electron microscopy and fluorescence spectrum analyses indicated that CC49-QDs had a0.3nm higher average diameter than the primary QDs, and that the grafted CC49had no obvious difference in optical properties compared to the primary QDs. Western Blot and immunohistochemical examination showed the existence of the TAG-72in the MGC80-3cell line.In vitro fluorescence imaging of the tumors showed that CC49-QDs probe could bind TAG-72expressed on MGC80-3cells.Conclusion:In conclusion, by the addition of the CC49, we generated a specific QDs molecule that not only has the potential to bind tumor cell in vitro, but also could be used in a long-term therapeutic regimen to possibly alter the cancer individual treatment. Further pre-clinical studies utilizing our CC49-QDs fusion construct, addressing the short-term and long-term capabilities, will be performed to develop regimens for improved gastric cancer treatment.