Expression Of Antigenic Epitope Of N - Terminal Domain Of Hepatitis B Virus Polymerase And Evaluation Of Its Antigenicity

Since HBV DNA polymerase has been detected in1970s, it has been well characterized.There is no doubt that such a product or products are expressed during infection in vivo, but the modes of transcription,translation and post-translational processing are not exactly known.Recombinant DNA technology,cloning and expression in E.coli protein,is a relatively technology.E.coli has a clear background easy to operate,rapid growth and high expression and yield adventages.The sequence of HBV-pol is derived from HBV genome recombinant plasmid by PCR,inverted into the expression vector of pET32a.The recombinant plasmid is then transported into BL21.After culture and induction.the target protein is available.HBV-pol was attached to the plate in ELISA and detection method is established.It proved that HBV-pol is a good indicator for the detection of chronic hepatitis.But more research is needed for HBV-pol.This project aim to establish a new method for the diagnoitic of hepatitis.