Study On The Changes Of Ovarian Hormone Expression And Differentially Expressed Genes In Rats By Exercise - Induced Estrous Cycle

Objective:The purpose of this study is to preliminary research the changes of the expression of related ovarian hormones and the differentially expressed genes of ovaries in rats with estrous inhibited resulting from long-term exhaustive swimming, then to discuss the Athletic menstrual cycle irregularities from receptor and gene level.Methods:22(3-months old) female Sprague-Dawley(SD)rats having normal estrous cycles were divided into two groups randomly:the control group (n=10), the experimental group (n=12).Rats of experimental group were trained by over-loading swimming for6days a week and1day for rest. Detection of desquamated keratinocyte index of vagina was done everyday for observation the changes on rat’s menstrual cycle. Finally, the all animals were killed and the ovaries were collected. Then the methods of HE staining and immunohistochemistry chemistry were used to observe ovarian general organizational structure, ultrastructure, ovarian E2, ER, PR, and serum reproductive hormones expression changes,And RIA technologies were used to test serum FSH, LH, E2, P concentration. By RFDD-PCR technology in the pre-test to establish two sets of gene expression profiles, and select the differential expression profile and protein degradation closely related to the ubiquitin-proteasome system genes such as Psmb5, Psmdl, Psmd8, Ube3a, UsplO, Usp9x quantitative PCR to verify.Results:1. In the training process, the movement of the control group rats Gradually appeared the estrous cycle disorders, will blank control rats to maintain normal estrous cycle.2.The results of HE staining show that:in control group, there were many follicles in different phases, especially typical growing follicles, and many fresh corpus luteum in the cortex of rat ovaries; compared with experimental groups, less typical growing follicles, mature follicles and fresh luteum corpus and relatively more primordial and atresia follicles can be observed.3、Ovarian E2, ER, PR immune group of analysis results:exercise group particles cells and luteal cells’E2expression IOD were lower than the control group significantly lower (P<0.05); compared to control group, model group’s ovarian granulosa cells and yellow body cells’IOD ER expression were lower (P<0.05); compared with the control group, the model group granulosa cells and luteal cells PR expression IOD significantly lower(P<0.05);4、 Serum reproductive hormone results:Compared with the control group, the model serum E2, P, FSH and LH levels were significantly lower (P<0.05), with a significant difference;5、after the validation of quantitative PCR, Psmb5, Psmdl, Psmd8, Ube3a, Usp10, Usp9x model group were overexpressed, consistent with the measured results RFDD-PCR. Conclusion:1.The long-term exhaustive exercise can cause suppression of the female rat estrous cycle, to provide animal models for the study of the AMI.2.Long-term exhaustive exercise in rats make ovarian morphological changes, thereby affact ovarian function, change the altered expression of ovarian hormones, and reduce serum levels of sex hormones;3.Psmb5, Psmdl, Psmd8, Ube3a, Usp10, Usp9x’s overexpression of the ubiquitin-proteasome system may play an important role in the female rat estrous cycle inhibition caused by long-term exhaustive exercise.