Study On Differentiation Of Chicken Primordial Germ Cells Into Spermatozoa In

Objective To construct the chicken primordial germ cell line and induce the cell differentiatedto sperm in vitro, to provide the theoretical foundation for curing the human infertility.Methods Conducting the biological characterization of chicken primordial germ cell line viausing RT-PCR, immunofluorescence technique, flow cytometry, embryoid bodies and teratomaformation protocol and other cell-molecular biological methods, induceing the cell linedifferentiating to sperm via retinoic acid and characterized the sperm.Results An stable chicken primordial germ cell line was constructed and characterized, formedembryoid bodies including one of a maximum diameter380μm, the chicken model of preparingteratoma was established, successfully obtained induced sperms with mobility.Conclusion The research on chicken primordial germ cell-induced sperm with mobility hasprovided an reliable animal model and protocols for the mechanism research on stem cell-induced sperm, which could also providing supporting materials promoting stem cell-inducedsperm have used in the clinic to treat the asthenozoospermia or the oligospermia and bringingnew hope for thousands of infertility patients.