| Interaction between microorganisms and soil minerals widely exists in the earth, which plays a great role in transmutation of nutrient element and organic pollutants in environment including water, soil and gas environment. Soil mineral plays a great role in soil, clay mineral are a active particles. Bacteria are most number in microorganisms. Interaction between microorganisms and clay minerals play a large role in soil physics, chemistry and biology. In the present paper, we selected clay minerals including kaolinite, smectite, and goethite and bacteria including Bacillus thuringiensis and Pseudomonas putida as the material to establish the method for study the interaction between bacteria and clay minerals and to study the effects of ambient factors on interaction between bacteria and clay minerals. The main results are as follows:1. Three methods for determining of concentration of proteins were used to determine the concentration of proteins in solution containing bacteria. As a result: Relationships between the concentration of proteins and the number of bacteria were established, which showed the relationship between the concentration of proteins and the number of bacteria was linear. Therefore, the number of bacteria in solution was used to replace by the concentration of proteins. |