Selection Of Recipients And Establishing Of Maize Inbred Lines Transformation By Agrobacterium-mediated

The genetic transformation of plants by the Gram-negative soil bacterium Agrobacterium tumefaciens is the only known example of inter-kingdom genetics exchange. This process is considered preferable to genetic transformation by artificial approaches (e.g., electroporation, microinjection, or biolistic bombardment of cells with highly accelerated naked DNA molecules) because of the ease and low cost of the procedure and because of the relatively low complexity of intact transgenes integrated into the plant genome. So Agrobacterium-Mediated Transformation is a major technique for genetic engineering of plants. The abundant study on the genetic transformation of maize proved that embryonic callus induced from immature embryo is perfect recipient. Genotype is considered as an important factor that affects transformation via Agrobacterium tumefactions. Although great advances have been made over the past decade to increase the number of plant species that can be transformed and regenerated using Agrobacterium by altering the bacterium, changing culture condition of recipient, optimizing system of transformation and so on, many important species or inbred lines remain highly recalcitrant to Agrobacterium-mediated transformation.In our research, ten maize inbred lines (6010, 6015, 6034, 6038, 6050, 6051, 6058, 6060, 6069 and 6077) have been selected from seventy maize inbred lines by immature embryo culturing, which had high induction frequency and clone ability of embryonic callus, could be subcultured in a long time and could obtain plant regeneration. The frequency of differentiation of inbred lines 6010, 6060, 6038, 6051 and 6015 was higher than that of the others. There were thirteen inbred lines(6002, 6005, 6022, 6024, 6029, 6043, 6048, 6061, 6070, 6071, 6074, 6078 and 6081) that couldn't induced embryonic callus in seventy inbred lines. So it could be proved that genotype is a key factor that determines production and differentiation of embryonic callus.