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Screen Fine Ganoderma Strains And Study On Immunological Activity Of Ganoderma Polysaccharides And The Optimization Submerged Fermentation Condition Of Xuezhi

Posted on:2007-03-07Degree:MasterType:Thesis
Country:ChinaCandidate:X Q HuangFull Text:PDF
GTID:2133360185980247Subject:Microbiology
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In this research, the cultural property , content of Ganoderma polysaccharides and triterpene, and the immunological activity of polysaccharides were combined to screen fine Ganoderma strains and comfirmed them best production mode in order to promote development of Ganodermaindustry.And studied on submerged fermentation condition of Xuezhi, the result would provid a theoretical reference of manufacture Xuezhi mycelium and metabolin by fermentation.All Ganoderma. sp were cultivated with basswood in plastic shed and classified four groups according to morph and size of spores,GroupI included Zi zhi,Heizhi,Huangjinzhi and Zilingzhi,GroupII inculded Xuezhi,GroupIII covered Meiguo dalingzhi and Jinda, and GroupIV covered all other strains. First all, we obtained the strains with excellent cultural property from different groups, covered Huangjinzhi, Heizhi,Xuezhi, Meiguodalingzhi, Jingda,Chizhi 6 hao, Jingdalingzhi, Songshalingzhi, Dechanglingzhil~#, Wubaolingzhi and Chizhi, on the basis of hypha growth state , fruiting body shape, spore producing rate, and growth cycle etc. Then , we had screened fine Ganoderma strains with rich Ganoderma polysaccharides or triterpene,The Xuezhi, Huangjinzhi, Meiguodalingzhi, , Dechanglingzhil~# Chizhi, Jingda and Songshalingzhi were included.We studied the influence of fruit body and mycelium polysaccharides of these strains with rich polysaccharides on immunological function in mice. By Aggregate analysising, The result had indicated that polysaccharides of Xuezhi mycelium and Huangjinzhi fruit body had significance effection on recovery humoral immunity of mice;all polysaccharides couldn't recovery cellular immunity and structural immunity except Meiguodalingzhi mycelium's for liver-spleen index;There wasn't necessary linkage between the contents of polysaccharides and pharmaco-activity; and there wasn't evident difference of immunological activity between fruit body and mycelium polysaccharides.The results of polysaccharides and triterpene contents and immunological...
Keywords/Search Tags:Ganoderma.sp, Cultural property, Polysaccharides, Triterpene, Immunological activity, Liquid fermentation
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