The Changes Of Glucosinolate Of Leaves In Different Stages In Brassic Napus L.

Glucosinolates of Brassica genus was an important secondary metabolites with many physiological functions, such as sulphur storage in seed, increase resistance to phytopathy microbe and insects. When the tissues of plant invade by disease or insect or injured, glucosinolates can be hydrolysised by myrosase, the hydrolysis products have a protective function. But high glucosinolates content (>40 μ mol/ g ) also affect the utilization of meals of rapeseed. There are more than twenty kinds of glucosinolates in rapeseed. The previous study indicated that glucosinolates content of the root, stem, leaf and seed of rapeseed controlled by different gene. In order to obtain high resistance in growth stages of double low rapeseed, it's possible to develop the rapeseed varieties with high glucosinolate content in leaves or stem but low glucosinolate content in seed.In this study, diferent methods were used in glucosinolate extraction from samples leaves: leaf drying with liquid nitrogen, lyophilization and heating method. The results showed that lyophilization was the most efficient with better repeatability and easy operation. Extracts of the glucosinolates with ultrasonic distiling, 75°C water bath distilling., triturator distill, results showed that ultrasonic distiling was the most efficient method with high distilling efficiency, short distilling time and less effects to the distilling component. Recovery efficiency of adding standard sample to the leaves of different glucosinolate content is more than 94%, the range is 94%~103%. The precision experiment is repeating the dispose ten times by using the same leaves. The average was 8.38 μ mol/g leaves powder, the difference between every sample and the average<2 μ mol/ g, and the difference between the high sample and the low sample < 3 μ mol/ g, the error was in the range of international standard, could be used in the detecting glucosinolate content of the leaves in Brassica napus L.The glucosinolates content of the leaves in Brassica napus L. was different in different growth stage. The glucosinolate content of the leaves kept high level in seedling stage and then decreaced,...