Transformation Of Gossypium Hirsutum Al-induced (GhAlin) Gene

Cotton (Gossypium hirsutum L.) is the most important natural fiber crop and vital for textile industry. Being the largest cotton producing and consuming country in the world, China consumes 1/3 amount of the global cotton consumption each year. Therefore, the cotton fiber production occupied an important position in the national economy. The traditional breeding methods have already can't meet the needs to the improvement of cotton fiber quality. Gene engineering, especially the plant genetic engineering, provide a high-performance approach to transformation of useful genes into cotton.The asparagine synthetase gene is not a house keeping gene, but is tissue specific expressed and regulated by a great deal of factors such as light and metabolism. During the development stage of cotton fiber, the plant consumed a lot of carbon element, so the rate of nitrogen to carbon increased, therefore, the plant is inclined to use asparagine for transportation and storage of nitrogen.The cotton GhAlin gene, which cDNA has a total of 1311 bp and an ORF of 711 bp, is obtained from a cotton ovule/fiber cDNA library by random sequencing. Sequence comparison showed that the GhAlin gene was highly homologous to the Al-induced protein gene (wali7) from wheat and the asparagine synthetase gene from Arabidopsis thaliana.We employ the agrobacterium-mediated transformation method to transform overexpression and anti-sense suppressing GhAlin gene into cotton. The main results are as follows:1. Choosing the agrobacterium -mediated transformation method using cotton hypocotyls for infection, we determined after 2 days co-cultivation, the best medium is MSB with 0.1mg/L 2,4-D and 0.5mg/LKT.2. The Northern blot analysis result showed that there has been a high expression of GhAlin in 12 DPA ovule, but without any expression in 12 DPA and 18 DPA fiber. This result suggests that...