This study had the objective of increasing the utilization of crude fiber by sheep even to increase the performance of sheep, and to discuss the proper supplementation of different sulfur sources and sulfur levels in sheep diet. Six Mongolian local sheep with permanent ruminal fistula were selected and randomly allocated into 2 groups (SA group and SB group), and sodium sulfate and sodium sulfide were used as the sources of sulfur of the two group respectively, each group were supplemented 3 sulfur level. Sulfur supplementation levels in ration (DM basic) were 0.12%(control), 0.18%(S-1), 0.25%(S-2), 0.30%(S-3) respectively, the basic diets were composed of corn straw and concentrate. The experiment was divided 4 periods to investigate the effect of different supplementation levels of sodium sulfate and sodium sulfide on the internal environment of rumen, the population of rumen microbe, the degradation of fiber substance in rumen and the nutrients digestibility in sheep. The result showed as follows:1. Sodium sulfate and sodium sulfide both could promoted the rumen fermentation, decreased the NH3-N concentration and increased the acetic acid, propionic acid and the concentration of total VFA in rumen.2. Sodium sulfate and sodium sulfide both could promoted the growth of rumen microbe, when the supplemented Sodium sulfate in diet reached diet dry matter content 0.25%, the average amount of fungi and bacterial in rumen had increased significantly, from 5.25×108/ml,8.31×103/ml to 16.09×108/ml,16.08×103/ml and 14.61×108/ml,11.06×103/ml respectively; When the sulfur content of diet dry matter reached 0.3%, the growth of rumen microbe had been restrained, the quantities of ciliates, bacteria and fungi all had decreased, and the synthesis of micro-protein had decreased.3. Sodium sulfate and sodium sulfide both could increase the rumen CMCase and the valid dynamic degradation of fiber substance significantly in rumen. With the increase of sulfur supplementation, the degradation of corn straw dry matter was increased gradually. When the sulfur content of diet dry matter was 0.18%, the valid dynamic degradation of NDF and ADF in corn straw was highest.4. Sodium sulfate and sodium sulfide both could increase the apparent digestibility of dry matter, organic matter and ADF in sheep.5. Sodium sulfate and sodium sulfide could increase the apparent digestibility and... |