| The chrysanthemum, plum, cymbidium and bamboo were called the four gentlemen in the flowers with great value of ornament. In the international cut flowers market, it standed the first place with the 30% total amounts of the cut flowers. Currently the production and supply of cut-off chrysanthemum in our country were lagged far behind, and hadn't become a whole set of industrial producing system. This research aimed to form a series of technique system through the method of rapid propagation in vitro from the beginning of cultivating seedlings, to decline cost, to guarantee the yield and quantities so as to conquer the large market of Japan even of Russia. The main results were as follows:1,Different antiseptics, different place of explants and period of sterilization could have great influence on the sample of cut-off chrysanthemum. The results indicated that the best effect of sterilization was to deal with alcohol of 70% density for 30 seconds and then with HgCl2 of 0.1% density, especially, 7min-long stem with axillary bud and 5min-long stem with terminal bud were better.2,Different concentration and kinds of hormone had greater influence on the inducement of buds. The combination of 6-BA/IBA (0.4~0.5/0.08~0.1 mg/L) led to larger quantity of buds and less callus would be the best. The alternate use of MS and MS+0.5mg/L 6-BA +0.01mg/L IBA had a better effect on the cultivation of the chrysanthemum cuttings. In addition, the cultivating temperature also had some influence on the growth of tube seedlings. The temperature of 24~26℃was the best, and the average propagation coefficient was 6.4.3,Different substrate had different effect on the cut-off chrysanthemum to take root. 1/2MS+NAA0.10mg/L and /2MS+NAA 0.15mg/L had the highest root-taking rate and the most roots. But from the aspect of economics, the best substrate was 1/2MS+NAA0.10mg/L comparing their growth condition of...
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