| The genetic linkage map constructed and integrated using new molecular markers still were the important contents of plant genome. Genetic linkage maps serve the plant geneticist in a number of ways, from marker assisted selection in plant improvement to map-based cloning in molecular genetic research. Soybean, a widely studied and economically important species, is derived from ancient tetraploid and strictly self-pollinated. Its genome contains a large amount of duplication areas and repetitive sequences. For these reasons the research of genetic mapping in soybean is dropped behind the other crops. Recently, SSR markers play a critical role in integrating maps from different populations.In this study, we used three populations for map constructing and integrating. Recombinant inbred population Jinf derived from the cross of the Jindou23 and ZDD2315, This population currently consists of 118 RILs; there are two F2 populations for map integrating, 03172 is an F2-derived population from the cross of Hajiao96-9 and ZYD677, 03197 is an F2-derived population from the cross of Heinong33 and ZDD2315. The parents of these populations are difference in many important agronomic traits. In the future, These facilities to locate the QTL of the important agronomic traits.513 pairs of SSR primers are used to screen polymorphism in randomly sampled population that contains 95 lines of 03172 map population. The results showed that 213 pairs of primers could amplify distinct bands between parents; the polymorphic rate was 41.5%. In 03197 map population, the results showed that 231 of 531 pairs of primers could amplify distinct bands between parents; it was found that the polymorphic rate was 43.5%. In a polymorphism rate point of view, SSR molecular have higher polymorphic rate in soybean than ISSR, AFLP, RFLP, RAPD which usually low 30%. In 03172 map population, 132 SSR primers were... |
